MAPK途径对玉米大斑病菌HT-毒素产生和生物学活性的调控作用  

Regulation of MAPK Signal Pathway on the Production and Biological Activity of HT-toxin in Setosphaeria turcica

作　　者：范永山[1] 谷守芹[1] 董金皋[1] 董娜[1] 

机构地区：[1]河北农业大学真菌毒素实验室

出　　处：《中国农业科学》2008年第1期86-92,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30471126);河北省科技厅博士资金项目(05547007D-2)

摘　　要：【目的】通过研究促分裂原活化蛋白酶(MAPK)途径对玉米大斑病菌HT-毒素产生及生物学活性的影响,探索该途径对玉米大斑病菌致病性的调控机制。【方法】利用MAPK途径特异性抑制剂U0126分析该途径对玉米大斑病菌HT-毒素产生及活性的影响。【结果】1～20μmol·L-1 U0126处理后,菌株01-23的HT-毒素组成和含量均发生了变化,部分组分的合成受到抑制,并诱导产生了一些新的毒素组分。1～20μmol·L-1 U0126处理后获得的HT-毒素在感病寄主B37和B37Ht1玉米自交系上的生物学活性都受到了显著抑制,在B37玉米叶片上的抑制程度强于B37Ht1。随着U0126浓度的增加,菌株01-23的HT-毒素在玉米叶片上产生的坏死斑面积减小,抑制程度增加,但不能完全抑制病斑产生,10μmol·L-1 U0126处理和20μmol·L-1 U0126处理间差异不显著。【结论】MAPK信号转导途径对玉米大斑病菌HT-毒素的产生和生物学活性具有一定的调控作用,但HT-毒素的产生和活性还受其它因素影响,具有更复杂的调控机制。[ Objective ] Elucidation of the effects of MAPK signal pathway on the HT-toxin production and biological activity of Setosphaeria turcica will facilitate the mechanism study of the fungal pathogenicity. [Method] The specific MAPK signal pathway inhibitor, U0126, was used to study the regulation of MAPK pathway on HT-toxin production and activity. [Result] The composition and quantity of HT-toxin fractions extracted from isolate 01-23 were significantly altered after treatment with 1-20 μmol·L^-1 U01262 The production or quantity of some fractions was inhibited and there are some new fractions were induced. The biological activities of HT-toxin on susceptible corn inbred lines, B37 and B37Ht1, were significantly inhibited in isolate 01-23 after treatment with in 1-20 μmol·L^-1 U0126. The inhibition was stronger on B37 than on B37Ht1. The inhibition increased from 1 μmol·L^-1 to 20 μmol·L^-1 U0126 treatments, but there was no significant difference between the 10 μmol·L^-1 and 20 μmol·L^-1 U0126 treatments, indicating that U0126 could not completely inhibit the biological activity of HT-toxin. [ Conclusion ] HT-toxin production and biological activity on susceptible corn leaves are regulated by MAPK signal transduction pathway, but the mechanism of HT-toxin production and biological activity in S. turcica is complex and infected by other unknown factors.

关 键 词：玉米大斑病菌 促分裂原活化蛋白酶 HT-毒素 U0126 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]