辣椒抗病基因同源序列的克隆与分析  

Cloning and Analysis of Resistance Gene Analogs from Pepper (Capsicum annuum L.)

作　　者：张丽英[1] 陈儒钢[1] 张俊红[2] 欧阳波[2] 肖景华[1] 李汉霞[2] 叶志彪[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070 [2]国家蔬菜改良中心华中分中心,武汉430070

出　　处：《中国农业科学》2008年第1期169-175,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助(30671416);教育部高等学校博士学科点专项科研基金资助(20050504028)

摘　　要：【目的】利用同源序列法分离辣椒抗病基因同源序列。【方法】根据已知植物抗病基因的NBS-LRR保守结构域设计简并引物,以辣椒品系PR205基因组DNA为模板对抗病基因同源序列进行扩增。【结果】获得了25个抗病基因同源序列(resistance gene analogs,RGAs),聚类分析将其分为7个不同的类别。由其核酸序列推导的氨基酸序列与Mi-1.2、prf、Hero、I2C-1、RPM1基因相应区域的同源性为27.4%～98.2%。其中RGA-p20与Mi-1.2基因的核苷酸和氨基酸序列相似性均达到99%,确认RGA-p20为抗根结线虫基因的一部分,即辣椒中也含有与Mi基因同源的根结线虫抗性基因。【结论】本研究在辣椒品系PR205中获得了抗病基因同源序列,为辣椒抗病基因的克隆奠定了基础。[ Objective ] Isolating resistance gene analogs from pepper using homology-based method. [ Method ]The resistance gene analogs from pepper （Capsicum annuum L.） line PR205 were amplified using degenerated primers based on the conserved regions of Nucleotide Binding Site （NBS）-Leucine Rich Repeat （LRR） domain from plant resistance genes reported. [Result] Twenty-five resistance gene analogs （RGAs） were obtained. These RGAs were classified into seven distinct groups by phylogenetic analysis. Putative amino acid sequences deduced from these 25 RGAs shared 27.4%-98.2% identity with the resistance genes of Mi-1.2, prf, Hero, I2C-1 and RPM1. RGA-p20 showes extremely high sequence identity （99%） with Mi-1.2 at nucleotide/amino acid levels, suggesting that RGA-p20 is one part of root knot nematode resistance gene in pepper line PR205 with high homology with Mi [Conclusion] In this study, twenty-five RGAs were obtained from pepper line PR205, which laid a promising foundation for cloning resistance genes from pepperr

关 键 词：辣椒 抗病基因同源序列 抗病基因 NBS—LRR 

分 类 号：S641.3[农业科学—蔬菜学]