猪-人混合淋巴细胞培养中细胞因子的检测  

The detection of cytokines in porcine-human mixed lymphocyte culture

作　　者：邵传森[1] 孙永良[1] 

机构地区：[1]浙江医科大学免疫教研室,杭州310006

出　　处：《中华器官移植杂志》1997年第3期158-160,共3页Chinese Journal of Organ Transplantation

摘　　要：为了探讨异种移植排斥反应与细胞因子的关系,检测猪-人混合淋巴细胞培养(MLC)中人类淋巴细胞的增殖反应及细胞因子含量,并与人类同种异体MLC进行比较。结果显示,前者增殖反应相当于或超过后者。两种MLC上清液中白细胞介素2和γ-干扰素的动态变化接近,且前者白细胞介素4的高峰值超过后者(P<0.05)。提示细胞因子在猪-人MLC中可能起着一定的作用。In order to explore the relationship between xenograft rejection and cytokines, the proliferative responses and cytokine contents from human lymphocyte were detected in porcine-human mixed lymphocyte culture (MLC) and compared with human allo-MLC. The results showed that the proliferative responses in the former was equal to or stronger than that in the latter, and the kinetics of IL-2 and IFN-γ in both supernatants were similar but the peak of IL-4 from the former was higher than that from the latter (P<0.05). This suggests that cytokines are likely to play roles in porcine-human MLC.

关 键 词：白细胞培养 混合型 器官移植 异体移植 淋巴细胞 

分 类 号：R617[医药卫生—外科学] R392.4[医药卫生—临床医学]