T型微通道装置制备尺寸均一壳聚糖微球  

Preparation of Uniform Size Chitosan Microspheres by a T-shaped Microchannel Device

作　　者：胡雪[1] 魏炜[1] 雷建都[1] 马光辉[1] 苏志国[1] 王化军[2] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100080 [2]北京科技大学土木与环境工程学院,北京100083

出　　处：《过程工程学报》2008年第1期130-134,共5页The Chinese Journal of Process Engineering

基　　金：国家自然科学青年基金资助项目(编号:20506026);国家高技术研究发展计划(863)基金资助项目(编号:2006AA10Z437)

摘　　要：采用T型微通道装置制备尺寸均一的壳聚糖微球.研究了乳化剂用量、油水两相流速比和流速等条件对乳液粒径的影响,尝试制备了不同分子量的壳聚糖乳液,并确定了交联固化方式.T型微通道装置的油相通道直径350μm,水相通道直径65μm,两通道接口处直径16μm.以1.5%(ω)的壳聚糖醋酸水溶液为水相,以液体石蜡/石油醚(7/5,φ)的混合物作为油相,水相流速20μL/min,油水两相流速比为15:1,4%(ω)的PO-500作为油相乳化剂,制备得到的壳聚糖乳液粒径分布系数<10%.以戊二醛的甲苯溶液作为交联剂,当戊二醛所含醛基与壳聚糖所含氨基的摩尔比为1:1时,交联时间选择2h.Uniform size chitosan microspheres were prepared by a novel T-shaped microchannel. The effects of emulsifier, different flow rate ratios and flow rates on the preparation of chitosan microspheres were studied, respectively. In addition, chitosan droplets with different molecular weights were prepared using the T-shaped microchannel device, and the effects of different glutaraldehyde solutions on cross-linking of microspheres were studied. The preparation conditions of chitosan microspheres were determined as follows： the diameter of oil phase channel in the T-shaped microchannel was 350 μm, the diameter of water phase channel was 65μm, and the joint diameter of two channel was 16 μm. 1.5%（ω） chitosan was dissolved in aqueous acetic acid, which was used as a water phase. A mixture of liquid paraffin and petroleum ether 7：5（φ） containing 4%（ω） PO-500 emulsifier was used as an oil phase, the flow rate of water phase was 20 μL/min, and the flow rate ratio ofoil to water phase was 15：1. The results show that the coefficient of variation （CV value） of all the chitosan droplets was below 10%. Glutaraldehyde toluene solution was finally chosen as the cross-linking agent of microspheres, the molar ratio of aldehyde group of glutaraldehyde to amino group of chitosan at 1：1 and the cross-linking time of glutaraldehyde 2 h.

关 键 词：微通道 微流控装置 壳聚糖微球 尺寸均一 制备 

分 类 号：R944.5[医药卫生—药剂学]