加拿大披碱草、肥披碱草及其杂种F_1 RAPD分析  

RAPD Analysis of Elymus canadensis,E.excelsus and their Hybrid F_1

作　　者：李小雷[1] 于卓[1] 贺鹏程[1] 马艳红[1] 李造哲[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019

出　　处：《草地学报》2008年第1期23-27,共5页Acta Agrestia Sinica

基　　金：国家自然科学基金(30560100);内蒙古自然科学基金(200607010505)资助项目

摘　　要：利用RAPD技术对加拿大披碱草（Elyrnus canadensis L．）和肥披碱草（Elymus excelsus Turcz．）及其杂种F1的遗传差异性进行研究，从153个随机引物中筛选出16个适宜引物，共扩增出215个RAPD位点，多态性位点比率为72．1％，平均每条引物的多态性位点为4．3～4．8个，DNA片段长度在100～2000bp之间。加拿大披碱草与肥披碱草的遗传距离较大（0．7122），杂种F1与父本肥披碱草的遗传距离较近（0．2329），而与母本加拿大披碱草相对较远（0．6643），杂种F，更偏向其父本。该结果可为加拿大披碱草一肥披碱草杂种后代的育性恢复和选育利用提供依据。The genetic diversity of Elymus canadensis L. , E. excelsus Turcz. , and their hybrid F1 were analyzed by the RAPD molecular marker technology. Total 215 loci were amplified from 16 primers out of 153 random primers, the polymorphic loci percentage were 72. 1%. The no. of band per primer ranged from 4.3 to 4.8, and the fragment length of the amplified products was from 100 to 2000 bp. The genetic distance between E. canadensis and E. excelsus reached to 0. 7122, while hybrid F1 was closer to to male E. ecccelsus （0. 2329） than female E. canadensis （0. 6643）, and hybrid F1 leaned to E. excelsus. These results could be used as foundation for the fertility restoration and utilization of the hybrid of E. canadensis and E. excelsus.

关 键 词：加拿大披碱草 肥披碱草 种间杂种 基因组DNA RAPD 

分 类 号：Q943[生物学—植物学]