可溶性Jagged1／Fc嵌合蛋白对小鼠淋巴细胞活化、增殖和周期的影响  

Effect of a soluble Jagged 1/Fc chimera protein on the activation,proliferation and cell cycle of lymphocytes in mice

作　　者：邢飞跃[1] 于哲[1] 刘静[2] 季煜华[1] 

机构地区：[1]暨南大学组织移植与免疫研究中心 [2]暨南大学医学院,广东广州510632

出　　处：《细胞与分子免疫学杂志》2008年第1期6-9,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30471635);广东省自然科学基金资助项目(04010451,5006033)

摘　　要：目的:研究可溶性Jagged 1/Fc嵌合蛋白在小鼠淋巴细胞活化、增殖和周期中的作用。方法:选择有效剂量为500μg/L Jagged 1/Fc嵌合蛋白(Jagged 1/Fc),以活体染料/羧基荧光素乙酰乙酸琥珀酰亚胺酯染色,建立在多克隆刺激剂刀豆蛋白A(ConA)或佛波醇酯(PDB)加离子霉素(Ion)刺激下评价淋巴细胞增殖的模型,通过流式细胞术分析Jagged 1/Fc对淋巴细胞增殖的作用;采用碘化丙锭染色检测Jagged 1/Fc对PDB加Ion刺激的淋巴细胞周期变化的影响;利用荧光标记的mAb双染技术观察Jagged 1/Fc对T细胞早期和中期活化标志CD69和CD25表达的影响。结果:Jagged1/Fc对ConA刺激或非刺激的CD3+细胞CD69和CD25的表达无明显影响,对ConA或PDB加Ion刺激或非刺激的淋巴细胞的增殖指数也无明显影响,导致非刺激的淋巴细胞sub-G0期细胞百分比增高,S期细胞百分比降低,但并不影响PDB加Ion诱导的淋巴细胞各期的变化。结论:这些结果提示500μg/L Jagged 1/Fc对小鼠淋巴细胞活化和增殖无明显作用,对PDB加Ion刺激的淋巴细胞周期也无明显影响,但能促进未受刺激的淋巴细胞凋亡,使细胞停滞于G0/G1期,阻止其进入S期。AIM： To investigate effect of a soluble Jagged 1/Fc chimera protein （Jagged 1/Fc） on activation, proliferation and cell cycles of lymphocytes in BALB/c mice. METHODS： A model to evaluate the lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A （ConA）, was established by a carboxy-fluorescein diacetate-succinimidyl ester （CFDA-SE）-Iabeling technique. Under an effective dose of 500 μg/L of Jagged 1/Fc, the effect of it on the lymphocyte proliferation was analyzed by flow cytometry. A propidium iodide-labeling technique was applied to estimate the influence of Jagged 1/Fc on the lymphocyte cell-cycle stimulated by phorbol 12, 13-dibutyrate （PDB） plus Ionomycin （Ion）. Expression levels of CD69 and CD2,5 molecules on surface of CD3＋ lymphocytes activated with ConA in the presence of Jagged 1/Fc were observed by a fluorescein-conjugated monoclonal antibody-labeling technique. RESULTS： Jagged 1/Fc had no effect on the expression levels of CD69 and CD2,5 of the CD3＋ lymphocytes stimulated with or without ConA, and no effect on the proliferation index of the lymphocytes stimulated by ConA or PDB plus Ion. It led to the increase of sub-G0 phase cell proportion and the decrease of S phase cell proportion of the lymphocytes, but did not influence the changes of the lymphocyte cell-cycle induced by PDB plus Ion. CONCLUSION： The results suggest that Jagged 1/Fc has no obvious effect on the activation and proliferation of lymphocytes in mice, but may promote the apoptosis of them, cause the G0/G1 phase arrest and block the S phase entry.

关 键 词：JAGGED 1 淋巴细胞 活化 增殖 细胞周期 

分 类 号：R392.11[医药卫生—免疫学]