抗汉滩病毒中和性鼠／人嵌合抗体基因转化拟南芥的初步研究  

Transformation of full-length gene of mouse/human chimeric antibody against Hantaan virus into Arabidopsis thaliana

作　　者：周伟[1] 吴兴安[1] 罗雯[2] 胡刚[1] 张芳琳[1] 白文涛[1] 张亮[1] 于澜[1] 史梦远[1] 徐志凯[1] 

机构地区：[1]第四军医大学微生物学教研室,陕西西安710032 [2]西安文理学院生命科学系,陕西西安710065

出　　处：《细胞与分子免疫学杂志》2008年第1期49-51,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30471626);全军医药卫生科研基金资助项目(06MA219);陕西省自然科学基金资助项目(2004C2-42)

摘　　要：目的:建立能表达抗汉滩病毒中和性鼠/人嵌合抗体基因的转基因植株,为汉坦病毒基因工程抗体的研究提供实验基础。方法:将构建的含抗汉滩病毒中和性鼠/人嵌合抗体基因的植物抗体表达质粒3G1MH-pCAMB IA2301,用TSS冻融法导入农杆菌GV3101,利用农杆菌介导的抽真空转基因技术转化野生型拟南芥,获得转基因植株。用PCR、Northernblot检测转基因植株。结果:经PCR分析表明,拟南芥的基因组中有抗汉滩病毒中和性鼠/人嵌合抗体基因整合,并成功获得7株T0代转基因拟南芥。植株提取RNA,经Northernblot分析可见约1 500 bp及800 bp处的目的条带,为编码重、轻链的目的基因。结论:成功地构建了含抗汉滩病毒中和性鼠/人嵌合抗体基因的拟南芥植株,为进一步利用植物表达治疗性抗体奠定了基础。AIM： To construct the transgenic Arabidop- sis thaliana containing full-length gene of mouse/human chimeric antibody （ 3G1MH ） against Hantaan virus. METHODS： The recombinant plasmid 3G1 MH-pCAMBIA2301 was transformed into Agrobacterium tumefaciens GV3101 by TSS freeze-thaw method, and then the recombinant was transferred into wild Arabidopsis thaliana by vacuum-transgenic method. The regenerated transgenic plants were selected with kanamycin, and confirmed by PCR and Northern blot. RESULTS： PCR result showed stable integration of the 3G1MH gene IN Arabidopsis thaliana genome in 7 stains of the transformed plants. Northern blot analyses confirmed the transcription of heavy and light chains in the transgenic plants. CONCLUSION： The successful establishment of 3G1 MH transgenic Arabidopsis thaliana plants pave the way for further research on expressing therapeutic antibody in transgenic plants.

关 键 词：汉滩病毒 嵌合抗体 转基因植物 植物抗体 

分 类 号：Q78[生物学—分子生物学] R373[医药卫生—病原生物学]