FK506对肝移植受者T细胞亚群上CD152和PD-1的调节研究  

Regulatory effect of FK506 on the expression of CD152 and PD-1 in the liver allo-recipients

作　　者：李佳[1] 白杨娟[1] 王兰兰[1] 卫红刚[1] 蔡蓓[1] 严律南[2] 吴泓[2] 

机构地区：[1]四川大学华西医院临床免疫实验室,四川成都610041 [2]四川大学华西医院肝移植中心,四川成都610041

出　　处：《细胞与分子免疫学杂志》2008年第1期54-57,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30670819);四川省科技攻关资助项目(2006Z09-037)

摘　　要：目的:动态观察肝移植受者外周血T细胞表面CT-LA-4/CD152和PD-1的表达,探讨FK506对负性协同刺激分子的调节作用。方法:采用酶增强免疫分析法测定FK506的血药浓度。外周血T细胞亚群和T细胞表面CD152和PD-1分子的表达利用流式细胞术(FCM)进行检测。结果:各时间组间FK506血药浓度无统计学意义(P>0.05)。随治疗时间延长,CD4+T细胞持续下降,至治疗3月时已明显低于健康对照组(P<0.05)。而各治疗组CD4+T细胞百分率均明显低于疾病对照组(P<0.05)。各时段治疗组CD8+T细胞均明显高于疾病对照组(P<0.05)。肝移植术后,CD4+和CD8+T细胞上CD152的表达较健康对照组升高(P<0.05),治疗2周和1月组还高于疾病对照组(P<0.05)。治疗2月和3月组CD4+CD152+T细胞的表达较治疗2周和1月组下降(P<0.05)。治疗3月组CD8+CD152+T细胞的表达较治疗2周和1月组降低(P<0.05)。术后T细胞亚群上PD-1的表达有升高趋势。CD4+T细胞上PD-1的表达在治疗2周开始明显高于健康对照组(P<0.05)。CD8+T细胞上PD-1的表达从治疗1月时明显高于疾病对照组(P<0.05)。结论:FK506能上调T细胞表面负性协同刺激分子CD152和PD-1的表达,可能参与抑制效应T细胞的过度增殖与活化,维持移植受者的存活和免疫稳态。AIM： To dynamically observe the expression of CTLA-4/CD152 and PD-I on T cell surface in the peripheral blood of liver allo-recipients, and to explore the regulatory effect of FK506 on negative costimulatory molecules. METHODS： The blood concentration of FK506 was measured by enzyme-multiplied immunoassay technique. Flow cytometry （FCM） was used to determine the expression of T cell subsets and CD152, PD-1 on T cell surface in the peripheral blood. RESULTS： There was no significant difference in blood concentration of FK506 between each group （ P〉 0.05）. With the progression of treatment, the frequency of CD4＋ T cells gradually decreased and was obviously lower than that in health control until the third month （P 〈 0. 05）. The expression of CD4＋T cells in each treatment group was significantly lower than that in disease control group （ P 〈 0.05 ）. And the frequency of CD8 ＋ T cells in each treatment group was obviously higher than that in disease control group （ P 〈 0.05 ）. After liver transplantation, the expression of CD152 on CO4＋ and CD8＋T cells was higher than that in health control group （P〈0.05）, and the expression in the second week and first month was higher than that in disease control group （ P 〈 0.05）. The frequencies of CD4＋ CD152＋ T cells in the second and third month were lower than that in the second week and first month （P〈0.05）. The frequency of CD8＋ CD152＋ T cells in the third month was lower than that in the second week and first month （ P 〈 0.05 ）. After liver transplantation, the expression of PD-1 on T cell subsets had the tendency of advancing. Its expression on CD4 ＋T cells was significantly higher than that in health control group from the second week （ P 〈 0.05 ）, and the expression on CD8＋T cells increased obviously from the first month compared with that in disease control group （P〈0.05）. CONCLUSION： FK506 could upregulate the expression of negative costimulatory molecules CD152 and PD-1 on

关 键 词：肝移植 CD152 PD-1 FKS06 

分 类 号：R392.4[医药卫生—免疫学]