依那西普对磨损颗粒诱导破骨细胞增殖的作用  

Effects of etanercept on wear debris-induced osteoclastogenesis in vivo

作　　者：张亮[1] 陈志荣[1] 吴兴临[1] 金群华[1] 

机构地区：[1]宁夏医学院附属医院骨三科,银川750004

出　　处：《国际骨科学杂志》2008年第1期57-59,共3页International Journal of Orthopaedics

基　　金：国家自然科学基金(30560155);教育部科学技术研究重点项目(206161)

摘　　要：目的探讨依那西普对关节磨损颗粒诱导体内破骨细胞增殖的影响,确定依那西普防治人工关节无菌性松动的可行性。方法60只BABL/C小鼠随机分为6组:A组为空白对照组,接受假手术;B～F组建立钛颗粒骨吸收模型,C～F组分别于0、2、4、6、8天腹腔注射不同剂量的依那西普(20μg/ kg、50μg/kg、100μg/kg、200μg/kg),10天后取出颅骨,进行病理学分析。结果HE染色中B组骨吸收明显增加,骨小梁结构紊乱及骨质疏松,破骨细胞的形态变大。数目变多;依那西普干预组上述表现减轻,尤以200μg/kg剂量为明显;TRAP染色中破骨细胞数目,A组为10.1±1.1个、B组为32.3±4.2个、C组为23.4±1.7个、D组为21.5±6.5个、E组为22.7±3.4个、F组为12.3±2.8个。与A组相比,钛颗粒组(B组)可明显增加破骨细胞生成(P<0.001);与B组相比,C～F组可减少破骨细胞生成(P<0.001),尤以200μg/kg剂量最为明显(P<0.001),与HE染色结果相符。结论依那西普可抑制破骨细胞的增殖,有望成为预防人工关节无菌性松动的药物。Objective To study the influence of etanercept in debris-induced osteoclastogenesis in vivo and evaluate the feasibility of etanercept for treatment of artificial joint aseptic loosening. Methods Sixty BABL/C mices were allocated into 6 groups. Group A was control group received sham operation, the bone resorption model of Ti-induced were established in group B to F, different doses of etanercept （20μg/kg, 50μg/kg, 100 μg/kg and 200μg/kg） were injected into abdomeinal in 0, 2, 4, 6, 8 days in group C to F, ten days later, mice calvariae were harvested for pathology analysis. Results In HE, bone resorption in group B increased obviously and the structure of bone trabicular showed disorderly and osteoporotic. The shape of osteoclast enlarged and the numbers of osteoclast increased. The findings was reduced in etanercept groups, especially in group F （200 μg/kg）. In TRAP, osteoclast numbers of osteolysis area in group A were 10. 1±1. 1, in group B were 32.3 ± 4.2, group C were 23. 4 ± 1.7, group D were 21.5±6. 5, group E were 22. 7±3. 4, group F were 12. 3±2. 8. Comparing with control group（A）, Ti particles increased osteolysis significantly （P〈0. 001）; comparing with group B, etanercept （group C to F） inhibited osteoclastogenesis effectively （P〈0. 001）, especially in group F （200μg/kg） （P〈0. 001）. The result was in agreement with HE stain. Conclusions Etanercept can inhibite wear particles induced osteoclastogenesis in vivo and hope to be a therapeutic candidate for the prevention of aseptic loosening.

关 键 词：依那西普 磨损颗粒 破骨细胞 人工关节 

分 类 号：R96[医药卫生—药理学]