中药912液对脓毒症大鼠心肌损伤保护的实验研究  

An experimental investigation on preventive effect of traditional Chinese medicine 912 solution(中药912液) on rats with septic myocardial injury

作　　者：庄海舟[1] 张淑文[2] 李昂[1] 段美丽[1] 沈潞华[3] 

机构地区：[1]首都医科大学附属北京友谊医院ICU,北京100050 [2]首都医科大学附属北京友谊医院感染内科,北京100050 [3]首都医科大学附属北京友谊医院心脏中心,北京100050

出　　处：《中国中西医结合急救杂志》2008年第1期16-19,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：北京市中医药管理局科研基金资助项目(A类165)

摘　　要：目的观察盲肠结扎穿孔术(CLP)脓毒症模型大鼠心肌损伤的发生情况,以及应用中药912液进行干预的效果及其对心肌的保护作用机制。方法采用CLP制备脓毒症大鼠模型;按照随机数字表法分组。共有56只存活Wistar大鼠进入该实验,其中假手术组8只,CLP模型组24只,中药912液治疗组24只,后两组再分为术后3、24和72 h 3个时间点亚组。观察各组不同时间点心肌损伤指标〔肌钙蛋白T(TnT)、脑钠素(BNP)〕,血清炎症因子水平〔肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、IL-10〕和血浆一氧化氮(NO)、丙二醛(MDA)的动态变化。结果CLP模型组大鼠术后3 h血浆TnT、TNF-α、IL-6、NO、MDA水平即开始较假手术组明显升高,BNP、IL-10到24 h时也明显升高(P<0.05或P<0.01);而中药912液组的TnT、BNP、TNF-α、IL-6、NO、MDA虽较假手术组升高,但明显低于CLP模型组,IL-10则高于CLP模型组(P<0.05或P<0.01)。结论脓毒症后可出现明显的心肌损伤,中药912液可通过抑制NO和自由基介导的组织损伤改善脓毒症时的心肌损伤。Objective To observe the occurrence of myocardial injury in rats with sepsis induced by cecum ligation perforation （CLP）, the intervention effect of traditional Chinese drug 912 solution（中药912液）and its mechanisms of protection on myocardial injury in rats with sepsis. Methods The models of rats with sepsis were prepared with CLP and randomly divided into groups. Fifty-six survival Wistar rats were taken into the experiment, including sham operation group （n=8）, CLP group （n=24） and 912 solution treatment group （n= 24）; the latter two groups each had three subgroups at three time points, 3, 24 and 72 hours. The dynamic changes of the indexes of myocardial injury were observed at various time points in the groups, including troponin T （TnT）,brain natrium peptide （BNP）, blood serum cytokines [tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6）, IL-10], plasma nitrogen monoxidum （NO） and malondialdehyde （MDA）. Results TnT, TNF-α,IL-6, NO and MDA in plasma were began to increase significantly in CLP group at 3 hours after CLP, hut BNP and IL-10 were increased at 24 hours compared with sham operation group （P〈0.05 or P〈0.01）, while such indexes （TnT, BNP, TNF-α, IL-6, NO and MDA） in 912 solution group were higher than those in the sham operation group, hut they were improved significantly compared with those in CLP group and IL-10 was significantly higher in 912 solution treatment group than that in CLP group （P〈0.05 or P〈0.01）. Conclusion： In sepsis, marked myocardial injury may occur, traditional Chinese medicine 912 solution can improve the injury by inhibiting NO and free radical mediated tissue injury.

关 键 词：脓毒症 心肌损伤 中药912液 炎症细胞因子 一氧化氮 丙二醛 

分 类 号：R285.5[医药卫生—中药学] R256.2[医药卫生—中医学]