大黄素对肠缺血/再灌注损害保护作用的实验研究  

Protective effects of emodin(大黄素) on intestinal ischemia/reperfusion injury in rats

作　　者：刘瑞林[1] 张嘉[1] 吴薇[1] 刘牧林[1] 

机构地区：[1]蚌埠医学院附属医院胃肠外科,安徽蚌埠233004

出　　处：《中国中西医结合急救杂志》2008年第1期45-47,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：安徽省医药卫生科研基金资助课题(2002A013)

摘　　要：目的探讨大鼠肠缺血/再灌注(I/R)损伤致肠黏膜损害的机制及大黄素的保护作用。方法将30只雄性Wistar大鼠随机分成假手术组、肠缺血45 min再灌注6 h模型组和大黄素预处理组。用无创伤动脉夹夹闭大鼠肠系膜上动脉制备肠I/R模型;假手术组仅开腹不钳夹血管。大黄素预处理组在术前30 min经股静脉注入大黄素(2.5 mg/kg),假手术组和模型组分别注入等量生理盐水。在缺血45 min再灌注6 h后,各组分别经下腔静脉采血,然后处死大鼠取肠系膜淋巴组织及小肠组织标本。分别检测各组血清肠脂肪酸结合蛋白(IFABP)、一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)含量;小肠组织丙二醛(MDA)、超氧化物歧化酶(SOD)、髓过氧化物酶(MPO)活性;血液及肠系膜淋巴组织细菌移位率;并进行小肠组织病理学观察。结果与模型组比较,大黄素预处理组IFABP、NO、TNF-α、MDA、MPO水平均明显降低(P均<0.01),SOD活性明显升高(P<0.01),肠系膜淋巴组织细菌移位率显著降低(血液2/10只比8/10只,肠系膜淋巴组织3/10只比8/10只,P均<0.05);病理损害明显减轻。结论大黄素可减少TNF-α、NO释放,抑制过度炎症反应,减轻中性粒细胞聚集及活化,减少大鼠氧自由基的生成,对大鼠肠I/R损伤具有保护作用。Objective To investigate the protective mechanism of emodin （大黄素）on intestinal mucosal injury induced by intestinal ischemia/reperfusion （I/R） in rats. Methods Thirty male Wistar rats were randomly divided into three groups, namely sham operation group （Group A）, group of ischemia 45 minutes followed by reperfusion 6 hours （Group B）, emodin-pretreated group （Group C）. The superior mesenteric artery was occluded and then released to produce the intestinal I/R model in rats. Group C was administrated emodin intravenously （2.5 mg/kg） before 30 minutes of the operation. In the sham operation group and model group, similar volume of normal saline was administered respectively. After 45 minutes of ischemia and 6 hours of reperfusion, the blood was collected from the inferior vena cava respectively in each group. Afterwards, the rats were sacrificed, and the mesenteric lymph node （MLN） and small intestinal tissues were taken for pathological analysis by light microscopy. The serum levels of intestinal fatty acid binding protein （IFABP）, nitrogen monoxidum （NO）, tumor necrosis factor-α （TNF-α）, and the activities of malondialdehyde （MDA）, superoxide dismutase （SOD）, myeloperoxidase （MPO） in the small intestinal tissues were measured, and the rates of bacterial translocation （BT） in blood and MLN were examined at 6 hours after reperfusion in each group. Results The contents of IFABP, NO, TNF-α, MDA and MPO were significantly lower （all P〈0.01）, SOD activity was significantly higher （P〈0.01） in group C than in group B. There was significant difference in the rate of BT between the group C and B （blood： 2/10 vs. 8/10 rats; MLN： 3/10 vs. 8/10 rats, both P〈0.05）. Histological examination displayed that the damage of intestinal mucosa was less in group C than in group B. Conclusion Intestinal I/R may result in intestinal mucosal injury, the release of abnormal TNF-a, NO, reactive oxygen and activated polymorphonuclear leucocyte （PMN） may

关 键 词：缺血/再灌注损伤 肠 大黄素 炎症介质 

分 类 号：R285[医药卫生—中药学]