钌(Ⅱ)酞菁自组装半胱胺酸电化学传感器  

Electrochemical Biosensor for Homocysteine in Serum by Ru(Ⅱ) Phthlocyanine Self-assembly Electrode

作　　者：干宁[1,2] 李天华[2] 王鲁雁[2] 

机构地区：[1]新型功能材料及其制备科学国家重点实验室,宁波大学材料科学与化工学院,宁波315211 [2]南京大学生命科学学院,南京

出　　处：《应用化学》2008年第2期189-193,共5页Chinese Journal of Applied Chemistry

基　　金：浙江省科技攻关项目(2006C31040);宁波市自然科学基金(2006A610048)资助项目

摘　　要：将四羧基钌(Ⅱ)酞菁(RuPc)共价键合到自组装在Au电极表面的巯基丁二胺单分子层上,获得了自组装单分子膜修饰金电极(RuPc-CME),并对其进行了IR、XPS表征。该修饰电极对L-半胱胺酸(Hcy)的氧化显示出较好的电催化响应,可用作Hcy的电化学传感器。探讨了传感器对Hcy的电催化氧化机理pH=5.5,考察了底液pH值的影响。传感器的计时电流曲线的响应时间小于5s,氧化电流与Hcy浓度在5.0×10-7～5.4×10-5mol/L范围内成良好线性关系,检测下限为1.8×10-7mol/L(S/N=3),可用于检测人血清样品中的Hcy。One monolayer containing thiol-tailed groups was formed via the covalent binding of Ru（ Ⅱ ） phthlocyanine（RuPc） on a dimercaptosuccinic amine-modified gold electrode by self-assembly, which was characterized by IR and XPS. The modified gold electrode showed an excellent electrocatalytic activity for the oxidation of homocysteine（Hcy） in a phosphate buffer solution （pH = 5.5 ）. The electrocatalytic mechanism and effect of pH of the buffer solution were also investigated. The steady-state current of chronoamperometry increased linearly with Hcy concentration in the range of 5.0 × 10^-7 ～ 5.4 × 10^-5 mol/L with a detection limit of 0. 18μmol/L（ S/N = 3 ）. The sensor also showed excellent reproducibility that makes it an ideal amperometric detector for Hcy in human serum.

关 键 词：金电极 自组装单分子层 电催化 钌(Ⅱ)酞菁 半胱胺酸 

分 类 号：O657.1[理学—分析化学]