硅胶修饰-表面分子印迹牛血红蛋白及其识别性能的研究  

Study of Recognition Property of Molecularly Imprinted Bovine Hemoglobin on Surface-modified Silica Gel

作　　者：刘秋叶[1] 李文友[1] 何锡文[1] 陈朗星[1] 张玉奎[1] 

机构地区：[1]南开大学化学学院,天津300071

出　　处：《化学学报》2008年第1期56-62,共7页Acta Chimica Sinica

基　　金：国家重点基础研究发展规划(973计划,No.2007CB914101);国家自然科学基金(No.20675040);天津市自然科学基金(Nos.06YFJMJC02800,07JCYBJC00500)资助项目

摘　　要：选用马来酸酐修饰的硅胶作为载体,丙烯酰胺为功能单体,N,N'-亚甲基双丙烯酰胺为交联剂,牛血红蛋白为模板分子,采用氧化还原悬浮聚合法,合成了具有选择性识别的牛血红蛋白分子印迹聚合物.并用红外光谱(IR)、扫描电子显微镜(SEM)和元素分析对聚合物进行了表征,结果表明载体表面成功地接枝了分子印迹聚合物薄层.同时选择性吸附实验表明分子印迹聚合物具有良好的识别性能,能实现水溶液中牛血红蛋白的富集.The silica modified with maleic anhydride was synthesized as a carder material. Then, molecularly imprinted polymers based on the surface-modified silica gel were prepared by redox polymerization of acrylamide with N,N′-methylene bisacrylamide as the functional monomer and the crosslinker in the presence of bovine hemoglobin as a template molecule. The results of IR and scanning electron microscopy demonstrated the formation of a grafted polymer layer on the modified silica gel surface. The adsorption studies also showed that the imprinted polymers had good adsorption capacity and specific recognition for bovine hemoglobin in an aqueous solution.

关 键 词：表面修饰分子印迹聚合物 牛血红蛋白 硅胶接枝 氧化还原聚合 

分 类 号：Q503[生物学—生物化学]