苏云金芽孢杆菌毒素Cry1Aa，Cry2Aa，Cry3Aa和Cry4Aa结构的计算机对比分析  

Comparison and Analysis of Cry1Aa,Cry2Aa,Cry3Aa and Cry4Aa of Bacillus thuringiensis Toxins with Computer

作　　者：赵新民[1] 夏立秋[1] 王发祥[1] 丁学知[1] 单世平[1] 张友明[1] 

机构地区：[1]湖南师范大学生命科学学院微生物分子生物学湖南省重点实验室

出　　处：《化学学报》2008年第1期108-111,共4页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos.30570050,30670052);国家“863”计划(No.2006AA02Z187)资助项目

摘　　要：运用生物信息学软件对苏云金芽孢杆菌毒素Cry1Aa,Cry2Aa,Cry3Aa和Cry4Aa的基本参数、一级结构、二级结构、三级结构、跨膜区和表面电势进行了预测比较.它们在一级结构上有较大差异,但二级结构和跨膜区相似,三级结构中各毒素的结构域I之间基本相似,结构域II之间差异较大,其中Cry2Aa为差异最大成员.4种毒素的表面电势分布不同.毒素之间的结构相似性和差异性与其作用机理和杀虫特异性有关.The primary parameters, secondary and tertiary structures and transmembrane regions of Bacillus thuringiensis toxins, Cry1Aa, Cry2Aa, Cry3Aa and Cry4Aa, were compared in bioinformatics. Considerable differences were found among the four toxins compared with each other in the primary structure, and many similarities were in the secondary structure and transmembrane regions. The structural diversity is smallest in domain I, domain II is the most divergent domain, and Cry2Aa is the most divergent member. The surface potentials over the four toxins were different. Those structural diversity and similarity may be involved in determining the action mode and insect specificity.

关 键 词：苏云金芽孢杆菌 毒素 结构 生物信息学 计算机 

分 类 号：Q93[生物学—微生物学] S476[农业科学—农业昆虫与害虫防治]