4个绵羊品种PTGS2基因部分片段的多态性分析  

Polymorphism analysis of partial fragment of PTGS2 gene in four sheep breeds

作　　者：王训翠[1] 储明星[2] 陈宏权[3] 朱国祺[1] 方丽[2] 叶素成[2] 

机构地区：[1]安徽中医学院科研处,合肥230038 [2]中国农业科学院北京畜牧兽医研究所中国农业科学院家养动物遗传资源与种质创新重点开放实验室,北京100094 [3]安徽农业大学动物科技学院,合肥230036

出　　处：《安徽农业大学学报》2008年第1期21-25,共5页Journal of Anhui Agricultural University

基　　金：国家863计划(2002AA211081);国家自然科学基金项目(30300248);北京市自然科学基金项目(5042017)共同资助

摘　　要：设计2对引物,采用PCR-SSCP和PCR-RFLP技术检测前列腺素内过氧化物合酶2(PTGS2)基因在小尾寒羊、湖羊、多赛特羊、萨福克羊4个绵羊品种304个个体中的单核苷酸多态性。结果表明,PTGS2基因引物1扩增片段在4个绵羊品种中不存在PCR-SSCP多态性。引物2扩增片段存在PCR-RFLP多态性,在4个绵羊品种中都检测到2种基因型(AA、AB),都没有检测到BB基因型。A等位基因频率明显高于B等位基因频率。小尾寒羊与多赛特羊、萨福克羊、湖羊之间PTGS2基因型分布差异显著(P<0.05),其余品种之间PTGS2基因型分布差异都不显著(P>0.05)。The single nucleotide polymorphism of prostaglandin-endoperoxide synthase 2 （PTGS2） gene was detected in four sheep breeds （Small Tail Han, Hu, Dorset and Suffolk） by PCR-SSCP and PCR-RFLP with two pairs of primers. The results indicated that there was no PCR-SSCP polymorphism in four sheep breeds detected by primer 1. There was PCR-RFLP polymorphism in four sheep breeds detected by primer 2. Two genotypes （AA and AB） were detected in four sheep breeds and BB genotype was not detected. Frequency of allele A was obviously higher than that of allele B. The PTGS2 genotype distributions were significantly different （ P 〈 0. 05 ） among Small Tail Han sheep and Dorset, Suffolk, Hu sheep.

关 键 词：绵羊 前列腺素内过氧化物合酶2基因 多态性 

分 类 号：S826.2[农业科学—畜牧学]