波尔-黄淮白山羊体细胞核移植重构胚电融合参数的选择  

Selection of the electrofusion parameter of reconstructed embryo from Bore-Huanghuai white goat somatic cell nuclear transfer

作　　者：李斌[1] 李东伟[1] 赵焕[1] 曹陈冲[1] 陈立子[1] 汪存利[1] 方俊顺[1] 韩伟[1] 刘健明[1] 陶勇[1] 章美林[1] 章孝荣[1] 

机构地区：[1]安徽农业大学动物科技学院

出　　处：《安徽农业大学学报》2008年第1期30-32,共3页Journal of Anhui Agricultural University

基　　金：安徽省"十五"重大科技专项(04003005);"十一五"科技攻关项目(06013051A)共同资助

摘　　要：以波尔山羊耳成纤维细胞为核供体细胞,黄淮白山羊卵母细胞为受体进行核移植,对重构卵进行电融合处理,发现电融合参数为250 V.mm-1、1次电脉冲时,40μs组的卵裂率显著高于10μs组和20μs组(P<0.05),但10μs组融合后的存活率最高(P<0.05)。当融合参数为250 V.mm-1、10μs时,2次电脉冲组的卵裂率显著高于1次电脉冲组(P<0.05),但1次电脉冲组的融合后存活率和胚胎获得率均高于2次脉冲组(P<0.05)。综合试验结果认为,适宜的山羊体细胞核移植的电融合参数是250 V.mm-1、10μs、1次电脉冲。In this study,cells nuclear transfer experiment was carried out using the ear fibroblasts of Bore goat as donor cells and the oocytes of Huanghuai-white goat as recipient cell. The result showed that when the electrofusion parameter was 250 V·mm^-1 for one DC pulse, the cleavage rate of oocytes with 4μ0 s electrofusion time, the electrofusion of reconstructed oocytes was significantly higher than that with 10 μs electrofusion time or 20 μs electrofusion time （P 〈 0. 05）, but the survival rate of the reconstructed embryos after the electrofusion with 10 μs electrofusion time was the highest （P 〈 0. 05 ） ; when the electrofusion parameter was 250 V·mm^-1 for 10 μs electrofusion time, the cleavage rate of reconstructed oocytes with two DC electrofusion pulses was significantly higher than that with one DC pulse, while the livability of post-electrofusion embryos and the rate of obtainable embryo in one DC pulse were both higher than those in two DC pulses （P 〈 0. 05 ）. It is the conclusion that the optimal electrofusion parameters for reconstructed oocytes of goat may be 250 V·mm^-1, 10 μs and one DC pulse.

关 键 词：山羊 核移植 电融合参数 

分 类 号：S827.36[农业科学—畜牧学]