荔枝核总皂苷体外抗乙型肝炎病毒的作用  

Inhibitory effects of total saponin extracted from the semen litchi against hepatitis B virus in vitro

作　　者：蒋蔚峰[1] 陈建宗[1] 张娟[1] 张金平[1] 贾新[1] 

机构地区：[1]第四军医大学西京医院中医药研究所,陕西西安710033

出　　处：《第四军医大学学报》2008年第2期100-103,共4页Journal of the Fourth Military Medical University

摘　　要：目的:观察荔枝核总皂苷体外抗乙型肝炎病毒(HBV)的作用.方法:以HepG2.2.15细胞为靶细胞,分别用不同浓度的荔枝核总皂苷和拉米夫定培养液培养,采用ELISA法及荧光定量PCR法测定细胞培养上清HBsAg,HBeAg及细胞外HBVDNA含量,评价荔枝核总皂苷对体外HBV的抑制作用.结果:荔枝核总皂苷对体外培养HepG2.2.15细胞分泌的HBsAg,HBeAg及细胞外HBVDNA的50%抑制浓度(IC50)分别为0.096,0.085和0.128g/L,治疗指数(TI)分别为6.98,7.88和5.23.0.25g/L拉米夫定组和0.4g/L荔枝核总皂苷组在d3,d6对体外培养HepG2.2.15细胞分泌的HB-sAg,HBeAg及细胞外HBVDNA的抑制作用差异无统计学意义(P>0.05).结论:荔枝核总皂苷具有一定的体外抗HBV作用.AIM： To evaluate the inhibitory effects of the total saponin extracted from the semen litchi against hepatitis B virus （HBV） in vitro. METHODS ： HepG2.2. 15 cells, as target cells, were exposed to different concentrations of total saponin and Lamivudine respectively. Enzyme-linked immunosorbent assay （ELISA） was used to assess HBsAg and HBeAg; fluorescent quantitative PCR was applied to quantify extracellular HBV DNA. RESULTS： The 50% inhibitory concentration （ICso） of total saponin against HBsAg, HBeAg and extracellular HBV DNA was 0. 096, 0. 085 and 0. 128 g/L respectively, and treatment index （TI） was 6.98, 7.88, 5.23 g/L respectively. The inhibitory effects of 0.25 g/L Lamivudine and 0.4 g/L total saponin against HBV had no significant difference between day 3 and day 6 （P 〉 0.05）. CONCLUSION ： The total saponin extracted from semen litchi inhibits HBV replication in vitro to some extent.

关 键 词：荔枝核 皂苷类 HEPG2.2.15细胞 肝炎病毒 乙型 

分 类 号：R512[医药卫生—内科学]