海马内微量注射褪黑素的免疫调节作用机制研究  

Study on the immunoregulatory mechanisms of melatonin microinjected into hippocampus in rats

作　　者：李俊[1] 汤晓林[1] 吴成义[1] 徐叔云[1] 

机构地区：[1]安徽医科大学临床药理研究所

出　　处：《中国药理学通报》1997年第3期220-222,共3页Chinese Pharmacological Bulletin

基　　金：安徽省教委自然科学基金

摘　　要：目的：进一步研究海马内微量注射（ｉｈ）褪黑素（ＭＴ）的免疫调节机制。方法：采用放射免疫法检测β－Ｅｎｄ和［３Ｈ］－ＴｄＲ参入法检测脾淋巴细胞增殖实验。结果：ｉｐ纳络酮（２．５ｍｇ·ｋｇ－１）能部分抑制ｉｈＭＴ的免疫调节作用，ｉｈＭＴ可明显提高下丘脑β－Ｅｎｄ水平，降低垂体的β－Ｅｎｄ。体外Ｎａｌ（０．２５ｎｍｏｌ·Ｌ－１～０．２５μｍｏｌ·Ｌ－１）对ｉｈＭＴ脾淋巴细胞增殖反应也呈剂量依赖性抑制作用，抗β－Ｅｎｄ抗体对ｉｈＭＴ增高的脾淋巴细胞增殖反应呈明显抑制作用。结论：ｉｈＭＴ的免疫调节作用可能主要与外周内源性阿片肽有关，而脾细胞产生的β－Ｅｎｄ可能在ｉｈＭＴ的免疫调节中发挥重要作用。AIM:To study the immunoregulatory mechanisms of melatonin(MT) microinjected into hippocampus (ih). METHODS: The levels of β endophin (β End) were measured by radioimmunoassay in different centeral nervous areas in ih MT rats, the proliferative responses of splenocytes were simultaneously conducted with TdR incorporation. RESULTS: The enhanced proliferations of splenocytes in ih MT rats were partly inhibited by naloxone (ip, 2 5 mg·kg -1 ) and the levels of β End were increased in the hypothalamus, however decreased in the pituitary. In vitro , the enhanced proliferation of splenocytes was also inhibited by Nal (0 25 nmol·L -1 ￣0 25 μmol·L -1 ) with dose dependent manner. The same actions as Nals were observed in splenocyte culture added anti β End serum. CONCLUSIONS:The immunoregulatory effects of MT(ih) could be related to the peripheral endogenous opioids, and β End produced by the splenocytes could have an important role in the immunoregulatory actions in ih MT rats.

关 键 词：海马 微量注射 褪黑素 免疫 内啡肽 

分 类 号：R977[医药卫生—药品] R967[医药卫生—药学]