基于胱胺自组装金纳米线的第三代过氧化氢生物传感器的研制  

Study on the tird gneration of hydrogen peroxide biosensor based on cystamine self-assembled Au nanowire

作　　者：杨光明[1] 白慧萍[1] 黄爱银[1] 卢旭晓[1] 杨云慧[1] 

机构地区：[1]云南师范大学化学化工学院,云南昆明650092

出　　处：《化学传感器》2007年第4期48-54,共7页Chemical Sensors

基　　金：云南省自然科学基金(No.2006B0028M);云南省教育厅自然科学基金项目(NO.07210087)

摘　　要：以聚碳酸酯为模板,采用电沉积法制得金纳米线,并利用胱胺将金纳米线自组装到金电极上,然后通过金纳米线吸附辣根过氧化物酶(HRP)制得新型无电子媒介过氧化氢生物传感器。考察了pH、电位等条件对传感器测定过氧化氢的影响。结果表明在pH6.90,电位为-0.110V条件下传感器检测过氧化氢的线性范围为2.0×10-4～2.8×10-2mol/L,该传感器稳定性好,制作简单。可用于实际样品中过氧化氢的测定,为过氧化氢的测定提供了一种新的手段。Au nanowires were produced by electrodeposition onto polycarbonate template and immobilized onto Au electrode modified with Cystamine Self - Assembled monolayer. Then, a mediatorless hydrogen peroxide biosensor is constructed based on adsorption of horseradish peroxidase onto Au nanowire, The effect ofpH and applied potential were investigated. The result shows that when the pH is 6. 90 and potential is - 0. 110 V, the currents increase linearly with the concentration of hydrogen peroxide in the range of 2.0 ×10^-4 - 2.8× 10^-2 mol/L, It is successfully used in the detection of hydrogen peroxide level with high stability, sensitivity,

关 键 词：胱胺 自组装 金纳米线 生物传感器 过氧化氢 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]