液相色谱-质谱联用法同时测定人血浆中雷米普利和雷米普利拉的浓度  

Determination of ramipril and ramiprilat in human plasma by HPLC-MS

作　　者：池玉梅[1] 李伟[1] 文红梅[1] 葛庆华[2] 王浩[2] 

机构地区：[1]南京中医药大学,江苏南京210029 [2]药物制剂国家工程研究中心,上海200437

出　　处：《中国医院药学杂志》2007年第12期1666-1668,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的：建立同时测定人血浆中雷米普利和雷米普利拉浓度的液相色谱一质谱方法。方法：以喹那普利为内标，血浆样品经固相萃取小柱提取后，采用HPLC-MS（TOF）联用技术，以电喷雾（GSI）作为接口技术，以宽的m/z为数据采集范围检测后，选择准分子离子[M＋H]^＋作为检测离子，进行滤过、积分的方式检测，同时测定人血浆中雷米普利和雷米普利拉的浓度。结果：雷米普利和雷米普利拉的线性范围分别为1～80μg·L^-1和0．5～40pμg·L^-1，日内精密度与日间RSD均小于10％。结论：该测定方法具有灵敏、专一和快速的优点，可满足雷米普利在人体内药动学研究的要求。OBJECTIVE To es.tabfish a sensitive and specific liquid chromatography-mass spectrometry （time-of-fly）[LC-MS （TOF） ] method for the determination of ramipril and ramiprilat in human plasma. METHODS Ramipril and ramiprilat and the internal standard quinapril were extracted from the human plasma using SPE column, then separated on a Symmetry C18 column The mobile phase consisted of methanol-acetic acid （47：53）, and was set at a flow rate of 0. 2 mL·min^-1. Detection was performed on a time-of-flight mass spectrometer equipped with an ESI interface and operated in positive-ionization mode. Ramipril quantity was realized by computing the peak area ratio （ramipril m/z 417/qunapril m/z 439）and ramiprilat quantity was realized by computing the peak area ratio （ramiprilat m/z 389/ qunapril rn/z 439） and comparing them with calibration curve （r = 0. 999 5）. RESULTS The linear calibration curve was obtained in the concentration range of 1-40 μg·L^-1 and 0. 5-40 μg·L^-1. The limit of quantitation was 1μg·L^-1 for ramipril and 0. 5 μg·L^-1. sions were less than 10%. CONCLUSION The method is sensitive, simple macokinetics study. for ramiprilat. Within-day and between-day preci and rapid for drug level monitoing in clinical pharmacokinetics study.

关 键 词：雷米普利 雷米普利拉 液相色谱-质谱联用 血浆 

分 类 号：R969[医药卫生—药理学]