FRO_2基因表达与铁还原酶活性及有效铁吸收的关系  

On the Relationship of Expression of FRO_2 Gene with Ferric Chelate Reductase Activity and Fe^(2+) Uptake

作　　者：武春霞[1] 邱苗苗[2] 杨静慧[1] 刘艳军[1] 黄俊轩[1] 梁国鲁[2] 

机构地区：[1]天津农学院园艺系,天津300384 [2]西南大学园艺园林学院,重庆400716

出　　处：《西南大学学报（自然科学版）》2007年第12期118-122,共5页Journal of Southwest University(Natural Science Edition)

基　　金：天津市自然科学基金资助项目(023614211;05YFJMJC14400);天津市科技发展规划资助项目05ZLZLZT01600;天津市农业科技成果转化与推广资助项目(0504018).

摘　　要：研究了转FRO2基因苹果突变体AFRO2的FRO2基因表达、铁还原酶活性和根系对有效铁的吸收.结果显示,FRO2基因在缺铁条件下大量表达;其表达量在根系中最强,其次是果实,叶片中最弱;在正常培养条件下,转基因AFRO2表达高于对照和其它非转基因品种;在缺铁胁迫下转基因AFRO2根系铁还原酶活性增加最多,吸收有效铁也最多.综合分析表明:FRO2基因表达量、铁还原酶活性、叶片有效铁含量3个指标有相同的变化规律,均为缺铁胁迫下最强.这说明,由于FRO2基因的转化和在八棱海棠苹果中的过量表达,使其编码的铁还原酶活性增加,根系还原和吸收的有效铁增多,致使其叶片中有效铁的积累最多.In order to understand the relationship between FRO2 gene and chlorosis resistance in apple plants, the expression of FRO2 gene, activity of ferric chelate reductase and reductive iron （Fe^2＋） contents in the roots were investigated. The results showed that FRO2 gene was over-expressedn under conditions of iron deficiency. The expression was the most in the roots, followed in sequence by the fruit and the leaves. The expression of FRO2 gene in the transgenic FRO2 apple （AFRO2） was higher than the CK and other varieties. The reductase activity and content of reductive iron increased in the roots under conditions of iron deficiency. Comprehensively considered, expression of FRO2 gene, reductase activity and Fe^2＋ content in the roots showed similar patterns in their changes. The activity of iron reductase coded by FRO2 gene increased owing to the over-expression of the gene. Finally, the roots deoxidized Fe more, so that a lot of effective Fe^2＋ was accumulated in the leaves.

关 键 词：转基因苹果 FRO2基因表达 铁胁迫 铁还原酶 有效铁 

分 类 号：Q786[生物学—分子生物学]