牛黄酸与维生素C对慢性砷暴露小鼠肝组织DNA损伤的保护作用  

Protective effects of taurine and vitamin C on liver DNA damage in mice chronically exposed to arsenic

作　　者：刘爽[1] 朴丰源[1] 曲淑贤[1] 孙鲜策[1] 姚晓峰[1] 叶建新[1] 李秋娟[1] 

机构地区：[1]大连医科大学卫生学教研室,辽宁省大连市116044

出　　处：《世界华人消化杂志》2007年第34期3567-3571,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金;No.3057158430600488;辽宁省教育厅资助项目;No.05L113~~

摘　　要：目的:观察牛黄酸与维生素C对慢性砷暴露小鼠肝组织DNA损伤的保护作用.方法:将小鼠40只随机分为4组,即:染砷组(4 ppm As_2O_3)、牛黄酸保护组(4 ppm As_2O_3)+150 mg/kg牛黄酸)、维生素C保护组(4 ppm As_2O_3+45 mg/kg维生素C)以及生理盐水对照组,用HE染色法进行组织病理学观察,用免疫组化及图像分析检测肝组织内8-OH-dG的水平.结果:染砷组小鼠肝组织出现胞质疏松化和气球样变等病理学改变,牛黄酸和维生素C保护组肝组织上述病理学变化较轻.免疫组化结果表明,染砷组肝组织呈现8-OH-dG的高表达(P<0.01).两保护组小鼠肝组织8-OH-dG的表达显著低于染砷组(P<0.01),其中牛黄酸保护组小鼠肝组织8-OH-dG免疫染色总光密度值(0.824±0.1062)显著低于维生素C保护组(246.7±61.81,P=0.012).结论:牛黄酸和维生素C对慢性砷暴露小鼠肝组织DNA损伤具有保护作用,其中牛黄酸的保护作用更明显.AIM： To observe the protective effects of taurine and vitamin C on liver DNA damage in mice chronically exposed to arsenic （As）.METHODS： Forty mice were divided into 4 groups： As group （4 ppm As2O3）, taurine protective group （4 ppm As2O3 ＋ 150 mg/kg tau- rine）, vitamin C protective group （4 ppm As2O3 ＋ 45 mg/kg vitamin C）, and normal saline group as controls. Liver tissues were obtained and HE staining was performed, 8-OH-dG expression was examined by immunohistochem-istry.RESULTS： Liver cells in mice in the As-treated groups showed swelling and ballooning degen-eration. 8-OH-dG was strongly expressed in these groups compared with the control group （P 〈 0.01）. The two protective groups showed mild damage with lower 8-OH-dG levels than the As group. The total optical density of 8-OH-dG immunohistochemical staining in the taurine protective group was lower than that in the vitamin C protective group （0.824±0.1062 vs 246.7± 61.81, P=0.012）. CONCLUSION： Mouse livers showed severe tissue injuries and DNA oxidative damage after long-term As exposure. Taurine and vitamin C alleviated this damage significantly, with the taurine group showing better recovery than the vitamin C group.

关 键 词：三氧化二砷 肝脏 8-羟基脱氧鸟嘌呤 牛黄酸 维生素C 免疫组化方法 

分 类 号：R575[医药卫生—消化系统] R595[医药卫生—内科学]