降脂益肝冲剂对NASH大鼠肝脏抵抗素mRNA表达的影响  

Effect of Jiangzhi Yigan Chongji on resistin mRNA in the liver of rats with non-alcoholic steatohepatitis

作　　者：杨文慧[1] 赵和平[2] 段晓燕[1] 刘婷[1] 葛姝囡[1] 

机构地区：[1]山西医科大学,山西省太原市030001 [2]山西医科大学第一临床附属医院综合科,山西省太原市030001

出　　处：《世界华人消化杂志》2007年第36期3798-3802,共5页World Chinese Journal of Digestology

摘　　要：目的:观察降脂益肝冲剂对非酒精性脂肪性肝炎(nonalcoholic steatohepatitis,NASH)大鼠肝脏抵抗素mRNA表达的影响,并探讨抵抗素在大鼠NASH发病机制中的作用.方法:24只♂Wistar大鼠随机分为3组:正常组普通饲料喂养,模型组和治疗组高脂饮食喂养.治疗组在高脂饮食12 wk后给予降脂益肝冲剂,同时模型组和正常组给予等量的生活饮用水灌胃,17 wk末处死各组大鼠.观察动物一般情况,肝组织脂肪变性及炎症活动程度,测定血清TNF-α、ALT、AST,空腹血糖(FBG)、空腹胰岛素(FINS),并计算胰岛素敏感指数(ISI);RT-PCR法检测肝组织抵抗素mRNA相对含量.结果:在正常肝脏组织探测到抵抗素mRNA的微量表达(0.42±0.08),模型组其表达量显著升高(2.14±0.11,P<0.01),与模型组相比治疗组则显著下降(0.90±0.06,P<0.01).模型组抵抗素水平与血清TNF-α、ALT、AST水平、肝脏炎症活动度计分成显著正相关(r=0.873,0.772,0.716,0.892,P<0.05),与ISI无相关.治疗组上述各项指标均得到显著改善.结论:抵抗素与反映肝脏炎症程度及肝功能的指标密切相关,可能在NASH的发病中起重要作用.降脂益肝冲剂对NASH有很好的疗效,抵抗素是其可能的作用靶点.AIM： To explore the effects of resistin in the pathogenesis of a rat model of non-alcoholic steatohepatitis （NASH）, and to observe the effect of Jiangzhi Yigan Chongji on resistin mRNA in the liver of that model, to explore its mechanism of action. METHODS： 24 male Wistar rats were randomly divided into three groups （eight rats in each）： normal group fed with standard diet, model group, and treated group fed with a high-fat diet. The treated group was given Jiangzhi Yigan Chongji （1 mL/100 g, its concentration is 0.8 g/mL, twice daily） by intragastric administration after 12 weeks. Meanwhile, the normal and model groups were separately given drinking water by the same means. After 17 weeks, they were killed. Resistin mRNA in the liver of the rat model of NASH induced by high-fat diet was detected with RT-PCR. Level of alanine aminotransferase （ALT） and asparate arninotransferase （AST） was detected by an automatic biochemistry analyzer; fasting blood glucose （FBG） was detected by glucose oxidase method; and tumor necrosis factor α （TNF-α） and fasting insulin （FINS） in serum were detected by radioimmunoassay. Degrees of steatosis and steatohepatitis were observed. RESULTS： A positive correlation was found between mRNA expression of resistin and TNF-α, ALT and AST, and the degree of steatohepatitis （r = 0.873, 0.772, 0.716 and 0.892, P 〈 0.05） in the model group, but had no correlation with ISI. The relative level of resistin mRNA in the liver tissue of the model group （2.14 ± 0.11） was significantly higher than that in the normal group （0.42 ± 0.08）, and that in the treated group （0.90 ± 0.06） was significantly lower than that in the model group （P 〈 0.01）. CONCLUSION： Resistin may play an important role in the damage of NASH. Jiangzhi Yigan Chongji can significantly decrease the expression of resistin mRNA in liver tissue of NASH rats, and resistin may be a pharmacological target.

关 键 词：非酒精性脂肪性肝炎 降脂益肝冲剂 抵抗素 大鼠 逆转录聚合酶链反应 

分 类 号：R285[医药卫生—中药学]