鲫血浆中甲磺酸达氟沙星的RP-HPLC法测定  

Determination of Danofloxacin Mesylate Concentration in Crucian Carp(Carassius auratus) Plasma by RP-HPLC

作　　者：潘玉善[1] 方之平[2] 操继跃[2] 王翔凌[2] 唐顺军[2] 潘黔生[3] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]华中农业大学动物医学院,湖北武汉430070 [3]华中农业大学水产学院,湖北武汉430070

出　　处：《河南农业大学学报》2007年第6期664-668,共5页Journal of Henan Agricultural University

基　　金：国家自然科学基金资助项目(30271013)

摘　　要：建立了测定鲫血浆中甲磺酸达氟沙星浓度的反相高效液相色谱法（RP-HPLC）.采用ZORBAX SB-C18色谱柱和SB-C18的保护柱;流动相：乙腈与磷酸盐缓冲液（含10 mmol·L^-1磷酸二氢钠和15 mmol.L-1四丁基溴化铵,磷酸调节pH4.0）的体积比为8∶92;流速：1.0 mL·min^-1;进样量：10μL;柱温：30℃;检测波长282 nm.内标物为盐酸氧氟沙星.本研究建立的甲磺酸达氟沙星标准曲线在0.05-5.00 mg·L^-1范围内呈良好的线性关系（R=0.999 9）,最低检测限为0.01 mg·L^-1,提取回收率均大于80%,日内及日间相对标准偏差小于11%.This study developed a method to detect danofloxacin mesylate（DFM） in crucian carp plasma using reversed-phase high performance liquid chromatography（RP-HPLC）.The reversed-phase analytical column ZORBAX SB-C18 was applied with guard column ZORBX SB-C18.The mobile phase used for the analysis consisted of acetonitrile and phosphate buffer（containing 10 mmol·L^-1 sodium dihydrogen orthophosphate and 15 mmol·L^-1 tetrabutylammonium bromide,adjusted to pH 4.0 with phosphoric acid）with ratio of 8∶92（V/V） and delivered at a flow rate of 1.0 mL·min^-1.The injection volume was 10 μL,with column temperature 30 ℃ and UVdetection wavelength 282 nm.Ofloxacin hydrochloride was selected as internal standard.Under these conditions,the calibration curve was linear in the range from 0.05 mg ·L^-1 to 5.00 mg· L^-1,showing better linear relationship （R=0.999 9）,with detection limit of 0.01 mg ·L^-1 and the average extraction recovery of more than 80%.The intra-day RSD and inter-day RSD were all less than 11%.

关 键 词：鲫 甲磺酸达氟沙星 内标 反相高效液相色谱法 

分 类 号：S948[农业科学—水产养殖]