高效液相色谱法测定抗骨增生丸中淫羊藿苷的含量  

Determination of Icariin in Kanggu Zengsheng Pills by HPLC

作　　者：施友玲[1] 

机构地区：[1]福建省药品检验所,福州350001

出　　处：《中国药品标准》2007年第6期61-63,共3页Drug Standards of China

摘　　要：目的:建立测定抗骨增生丸中有效成分淫羊藿苷含量的方法。方法:采用高效液相色谱法测定。用Hypersil BDS C_(18) (4.6 mm×250 mm,5μm)柱,以乙腈-0.1%磷酸溶液(28:72)为流动相,检测波长为270 nm,柱温为室温。结果:淫羊藿苷在0.030752～1.5376μg范围内呈良好线性关系,回归方程为Y=2227862.0X-2893.6(r=1.0000),淫羊藿苷的平均回收率为98.68%(RSD=1.11%,n=6)。结论:方法简便,分离效果好,结果准确可靠,可以用于抗骨增生丸的质量控制。Objective：To establish a method for determination of Icafiin in Kanggu Zengsheng Pills. Methods：HPLC method was carred on a column of Hypersil BDS C18 （4. 6 mm× 250 mm,5μm）with a mobile phase of methanol-0.1% phosphoric acid（28：72）. The detection wavelength was at 270 nm. Results：The linear regression equation was Y = 2 227 866. 0X -2 893.6 （r = 1.00 0） and the linear range was 0. 030 752 - 1. 537 6μg. The average recovery was 98. 68% （ RSD = 1.11 ,n =6）for Icariin. Conclusion：This is a rapid, simple and accurate method which can be used to control the quality of Kanggu Zengsheng Pills.

关 键 词：抗骨增生丸 淫羊藿苷 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学] R291.2[医药卫生—中医学]