比色法测定复方氨基酸注射液(18AA-Ⅰ)中焦亚硫酸钠的含量  

Content Determination of Sodium Pyrosulfite in Compound Amino Acid Injection(18AA-Ⅰ) by the Colorimetric Method

作　　者：吴小曼[1] 纪宇[1] 

机构地区：[1]江苏省药品检验所,南京210008

出　　处：《中国药品标准》2007年第5期38-40,共3页Drug Standards of China

摘　　要：目的:测定复方氨基酸注射液(18AA-I)中焦亚硫酸钠的含量。方法:二氧化硫能使酸性品红溶液褪色,供试液在室温放置25分钟,采用比色法在549nm波长处测定吸光度,用标准曲线法测定含量。结果:无水亚硫酸钠在3～21μg/ml范围内,吸光度的对数与浓度的对数呈良好的线性关系(r>0.9995):平均加样回收率为100.8%,RSD为1.6%。结论:本法操作方便,结果准确可靠,能满足复方氨基酸注射液(18AA-I)中焦亚硫酸钠含量测定的要求。Objective： Determination of sodium pyrosulfite in Compound Amino Acid Injection （18AA-Ⅰ） by colorimetric method. Methods：Sulfur dioxide released from sodium pyrosulfite can fade fuchsin acid solution. After standing at room temperature for 25 minutes,sample solution was determined at the wavelength of 549nm. The amount of sodium pyrosulfite in sample was calculated by reference to a calibration curve obtained, under the same condition,for known amount of sodium sulrite. Results：Sodium sulfite showed good linearity （r〉0. 9995）in the range of 3-21μg/ml. The average recovery was 100.8 with RSD 1.6% （n= 9）. Conclusion：The method is simple ,rapid and accurate,suitable for the determination of sodium pyrosulfite in injection.

关 键 词：复方氨基酸注射液 焦亚硫酸钠 比色法 含量测定 

分 类 号：R977.4[医药卫生—药品] R446.11[医药卫生—药学]