毒源和寄主对褐色桔蚜传播柑桔衰退病毒效率的影响  

Effect of virus sources and host plants on the transmissibility of Citrus tristeza virus by single aphid of Toxoptera citricida

作　　者：周彦[1] 周常勇[1] 王雪峰[1] 刘科宏[1] 宋震[1] 李中安[1] 

机构地区：[1]西南大学柑桔研究所/中国农业科学院柑桔研究所,重庆400712

出　　处：《植物保护学报》2007年第6期597-600,共4页Journal of Plant Protection

基　　金：国家自然科学基金资助项目(30471205);重庆市科委攻关专项(CSTC;2005AB1010)

摘　　要：褐色桔蚜是柑桔衰退病毒(Citrus tristeza virus,CTV)最有效的传播媒介,为了解不同柑桔寄主种类和毒源对褐色桔蚜传播CTV效率的影响,检测在以锦橙、凤凰柚和墨西哥来檬作毒源植株时,褐色桔蚜对10个CTV分离株的单蚜传毒率,以及蚜传前后CTV分离株p25/HinfⅠRFLP组群构成的变化,并对其中5个分离株与2个国外分离株进行了p20和p25基因相应氨基酸序列的比对分析。结果表明:褐色桔蚜传播CTV的能力受柑桔寄主种类的影响较大,以锦橙作毒源植株可以获得最高的传毒率;CTV毒株强弱以及褐色桔蚜虫态(有翅或无翅蚜)对单蚜传毒率影响不明显;褐色桔蚜传播具有p25/HinfⅠRFLP第3组群构成的CTV分离株的能力较强。Toxoptera citricida is the most efficient vector of Citrus tristeza virus （ CTV）. For better understand of the effect of CTV isolates and host plants on the transmissibility of CTV by single aphid of T. citricida, the transmission rates of 4 severe CTV isolates and 6 mild ones were evaluated, as Jincheng, Feng-huang pummelo and Mexican lime were used as host plants. And p20 and p25 proteins of 7 CTV isolates were compared, and p25/Hinf Ⅰ restriction fragment length polymorphism （RFLP） groups assay was used to analyze for 10 CTV isolates and 161 aphid transmission sub-isolates which obtained by transmitted from Jincheng to Jincheng, The results indicated that Jincheng was a better host for CTV transmission by T. citricida than that of Feng-huang pummelo and Mexican lime. The transmission rate of severe CTV isolates and mild ones were similar. In this study, CTV isolates with p25/Hinf Ⅰ RFLP group 3 had higher transmissibifity by T. citricida than the others.

关 键 词：柑桔衰退病毒 柑桔种类 单蚜传毒率 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]