春夏大白菜黑斑病病原鉴定和抗性鉴定方法比较  

Pathogen identification of black leaf spot in spring summer Chinese cabbage and comparison of the resistance identification methods

作　　者：王风敏[1] 张鲁刚[1] 刘静[1] 张华敏[1] 惠麦侠[1] 张明科[1] 

机构地区：[1]西北农林科技大学园艺学院农业部西北园艺植物种质资源与遗传改良重点开放实验室,陕西杨凌712100

出　　处：《植物保护学报》2007年第6期614-618,共5页Journal of Plant Protection

基　　金：国家科技支撑计划"优质高产白菜育种技术研究及新品种选育"(2006BAD01A7-1-03);陕西省13115工程"优质多抗专用蔬菜育种技术研究"(2007ZDKG-05)

摘　　要：为大白菜抗黑斑病新品种选育和种质创新,对陕西省春夏大白菜黑斑病进行调查、病样采集、病菌分离及形态和分子鉴定。结果表明,甘蓝链格孢Alternaria brassicicola是唯一病原。利用3个不同抗性的白菜品种研究了孢子浓度、苗龄和温度对大白菜黑斑病发病的影响,建立了大白菜抗甘蓝链格孢黑斑病的人工接种鉴定方法,即采用二叶期幼苗,以1×104pfu/mL孢子浓度喷雾接种,随后置25℃条件下,先黑暗下保湿24h,接着正常管理3天,从第4天开始夜间保湿,白天揭开,第8天保湿24h后调查发病情况,其结果客观反映大白菜品种的抗病性能。In order to provide technical support for breeding new varieties and germplasm innovation of Chinese cabbage resistance to black spot, me black spot leaves samples of Chinese cabbage were collected in Shaanxi Province. The pathogen was isolated and purified by single spore and identified by special molecule marker. It was determined that Alternaria brassicicola was the only pathogen causing black spot in spring-summer Chinese cabbage. By studying the influence of inoculation spore concentrations, seedling stage and growing temperature on black spot occurrence with three different resistance varieties, an artificial inoculation method for black spot identification was optimized and set up, which key procedure is praying 1 × 10^4 pfu/mL spores on seedling with two true leaves, and placing at 25℃ condition, then keeping moisture and dark for 24 h, keeping moisture at night from 4^th -7^th day, keeping moisture 24 h in 8^th day, then investigated. The result from the method was exact in accordance with the true resistance type of Chinese cabbage.

关 键 词：大白菜 黑斑病 甘蓝链格孢 抗性鉴定 

分 类 号：S436.341[农业科学—农业昆虫与害虫防治]