阿魏酸钠与牛血清白蛋白结合作用的研究  

Study on the Interaction between Sodium Ferulate and Bovine Serum Albumin

作　　者：张爱平[1] 王丽[1] 杨锦艳[1] 杨斌盛[2] 

机构地区：[1]山西医科大学药学院,太原市新建南路56号030001 [2]山西大学分子科学研究所,太原市030006

出　　处：《光谱实验室》2007年第6期1161-1165,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金(20371031)资助项目

摘　　要：用荧光、紫外光谱法研究了在模拟人体生理条件下阿魏酸钠与牛血清白蛋白(BSA)之间的相互作用,求得阿魏酸钠与BSA的结合常数K=(5.30±0.18)×105,根据热力学参数确定了阿魏酸钠与BSA之间的作用力类型,根据Foster偶极-偶极非辐射能量转移原理,计算了阿魏酸钠与BSA相互结合时其授体-受体间的距离r=1.01nm。The interaction between sodium ferulate and bovine serum albumin was studied by fluorescence spectroscopy and ultraviolet spectroscopy under the simulated physiological conditions the combining constant was obtained as K=（5.30±0.18）×10^5 by fluorescence quenching method.The type of interaction force between sodium ferulate and bovine serum albumin was determined by the thermodynamic parameters according to the theory of Foster＇s dipole-dipole non-radiation energy transfer mechanism,the binding distance（r=1.01nm）between sodium ferulate and bovine serum albumin was obtained.

关 键 词：阿魏酸钠 牛血清白蛋白 荧光猝灭 

分 类 号：O657.32[理学—分析化学]