珍珠黄杨两个天然群体遗传多样性的RAPD分析  

Analysis of genetic diversity of two populations of Buxus sinica var. parvifolia by RAPD

作　　者：姜自红[1] 季孔庶[1] 黄焱[1] 

机构地区：[1]南京林业大学,国家林业局、江苏省林木遗传与基因工程重点开放实验室,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2008年第1期11-14,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金资助项目(30070633)

摘　　要：以珍珠黄杨为材料,研究了RAPD分析过程中的模板DNA、Mg2+、随机引物、dNTP、TaqDNA聚合酶等影响因素,建立了适于珍珠黄杨RAPD分析的PCR反应体系,并在此基础上对珍珠黄杨两个天然群体的遗传多样性进行了分析。从200个随机引物中筛选出14个引物共检测出个161个位点,其中多态位点137个。用POPGEN32版软件对数据进行了分析,结果表明:多态位点百分率为85·09%,Shannon’s信息指数为0·5492,Nei’s基因多样性为0·3711,珍珠黄杨具有较高的遗传多样性。The effect factors such as the concentration of template DNA, Mg^2＋,dNTP,TaqDNA polymerase were selected in RAPD analysis. PCR system for RAPD in B. sinica var. parvifolia was set up. Genetic diversity of two populations of B. sinica vat. parvifolia was analyzed. There were 137 polymorphic loci among 161 RAPD loci of 14 primes selected from 200 random primers. POPGENE 32 was used to analyze the data. The results showed that the percentage of polymorphic loci was 85.09%. The Nei＇ s gene diversity and Shannon diversity index were 0. 371 1 and 0. 549 2 in the species respectively. These data indicated that B. sinica var. parvifolia had a relatively high lever of genetic diversity.

关 键 词：珍珠黄杨 RAPD反应体系 群体遗传多样性 

分 类 号：S722[农业科学—林木遗传育种]