补充活性肽对大强度离心运动后大鼠骨骼肌肝细胞生长因子表达的影响  

Effects of Soy Hydrolyzed Peptides Supplementation on Expression of Hepatocyte Growth Factor in Skeletal Muscle after Eccentric Exercise in Rats

作　　者：李世成[1] 李跃纲 王启荣[3] 杨则宜[3] 李肃反[3] 

机构地区：[1]湛江师范学院体育科学学院,广东湛江524048 [2]北京市疾病控制中心,北京100025 [3]国家体育总局运动医学研究所运动营养中心,北京100029

出　　处：《西安体育学院学报》2008年第1期53-57,共5页Journal of Xi'an Physical Education University

基　　金：科技部奥运科技专项资金资助项目(2002BA904B16)

摘　　要：目的观察补充活性肽对大鼠骨骼肌肝细胞生长因子表达的影响,研究活性肽促进运动性骨骼肌微损伤修复的机制。方法雄性SD大鼠40只随机分为补充安慰剂安静组、运动组和补充活性肽安静组、运动组,所有动物膳食平衡1 w后,进行实验。运动组大鼠以20±1 m/min的速度,坡度为-16°,持续性跑台训练120 min。实验组和对照组所有动物在膳食平衡期间每天分别进行灌胃15%活性肽饮料2 ml和等量的安慰剂。结果运动可刺激骨骼肌卫星细胞的激活和肝细胞生长因子的表达,而补充活性肽可能对肌卫星细胞的激活有正性作用,可显著增加大鼠离心运动后骨骼肌肝细胞生长因子的表达。Objective： Through immunochemistiy methods, the effects of soy hydrolyzed peptides supplementation on expression of HGF in skeletal muscle after intensive training were investigated. Methods： 40 Male Sprague-Dawley rats were randomly divided into 4 groups as placebo, SHPs supplemented and exercise or not. Rats ran 120 minutes at speed of 20＋ 1m/min and - 16°. Animals in control and experimental groups were fed 2 ml beverage containing 15% SHPs and placebo respectively. Results： The results showed that the immunostaining for HGF increased and activating muscle satellite cell following a bout of eccentric exercise, and after SHPs supplementation, the perentage of positive cell for HGF was the higher following exercise, and SHPs supplementation maybe up - regulate activating muscle satellite cell following a bout of eccentric exercise.

关 键 词：活性肽 骨骼肌 卫星细胞 肝细胞生长因子 离心运动 大鼠 

分 类 号：G804.7[文化科学—运动人体科学]