粘类小麦雄性不育基因rfv1定向导入的研究  

Directional Transduction of Male Sterile Gene rfv1 of NIAN Type in Wheat

作　　者：曹栎[1] 张改生[1] 牛娜[1] 张瑜[1] 位芳[1] 

机构地区：[1]西北农林科技大学陕西省作物杂种优势研究与利用重点实验室/小麦育种教育部工程研究中心,陕西杨凌712100

出　　处：《麦类作物学报》2008年第1期21-24,共4页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(301705760);国家"863"重大专项(2002AA207004);杨凌农业生物技术育种中心专项基金(99-1A);西北农林科技大学拔尖人才支持计划项目

摘　　要：为了研究粘类非1BL/1RS小麦雄性不育基因rfv1定向导入的途径与方法,从而创制粘类非1BL/1RS小麦雄性不育系的新保持系,以非1BL/1RS普通小麦变种莫迦小麦为供体材料,综合农艺性状优良的1BL/1RS易位系(普通小麦品种)西农Fp1为受体材料,采用定向回交置换方法,同时结合根尖染色体镜检和SDS-PAGE检测,将莫迦小麦核基因组中的rfv1不育基因定向导入到西农Fp1的核基因组,完成育性染色体的专一替换,育成既携有来自莫迦小麦核基因组的rfv1不育基因,又具有西农Fp1核背景的粘类非1BL/1RS小麦雄性不育系的新保持系。该方法为杂种优势利用中更好地发挥粘类小麦雄性不育系的实际作用奠定了坚实的技术支撑。In order to find a method that the new maintenance line with the male sterile gene rfv1 of NIAN type can be bred in wheat, the variety Xinong Fp1, a 1BL/1RS translocation line used as the acceptor and Triticum macha vat, subletschchumicum, non-1BL/1RS translocation line used as the donor, the directional substitution backcross was made by a observation of the chromosomes of root tip and the SDS-PAGE analysis. The male sterile gene rfv1 of Triticum macha vat. subletschchumicum was transduced to the genome of Xinong Fp1, the new maintenance line with the male sterile gene rfv1 of NIAN type was bred. This method provided a good way for breeding new male sterile type for hybrid wheat utilization.

关 键 词：小麦 粘类不育系 1BL/1RS易位染色体 rfv1基因 定向替换 

分 类 号：S512.1[农业科学—作物学] S330