枯草芽孢杆菌JA脂肽类及挥发性物质抑菌效应的研究  

Antagonistic Properties of Lipopeptides and Volatiles Produced by Bacillus subtilis JA

作　　者：陈华[1] 郑之明[1] 余增亮[1] 

机构地区：[1]中国科学院离子束生物工程学重点实验室,合肥230031

出　　处：《微生物学通报》2008年第1期1-4,共4页Microbiology China

基　　金：国家自然科学基金(No.20576132)项目资助

摘　　要：枯草芽孢杆菌JA产生的脂肽类抗生素对植物病原真菌有广谱抗性。将发酵液经过酸沉淀、甲醇抽提以及反相高效液相色谱等步骤,分离得到脂肽类抗生素的纯品。经IC_(50)实验和抗菌谱测定,考察了脂肽类抗生素对多种植物病原菌的作用,确定了脂肽类抗生素的抗菌谱。深入研究表明,枯草芽孢杆菌JA还产生未知成分的挥发性抑菌物质,能够抑制灰霉病菌孢子的萌发和菌丝的生长。脂肽类抗生素和挥发性抑菌物质的协同作用,有助于提高枯草芽孢杆菌的生物防治效果。Lipopeptides produced by Bacillus subtilis JA antagonized a broad spectrum of fungal pathogens. Crude lipopeptides were extracted with methanol from the precipitate which was obtained by adding 6 mol/L HCl to the cell-free culture broth. The crude extract was run on Diamonsil C18 column （5 μm, 250 mm×4.6 mm） in reverse phase HPLC system to purify the lipopeptides. Inhibitory ability and IC50 values of lipopeptides towards various microorganisms were determined by agar diffusion method. The results showed lipopeptides exhibited strong inhibitory activity against some important plant pathogenic fungi, including R. solani and E oxysporum. The ability of B. subtilis JA to antagonize against the growth of the post-harvest pathogen-B, cinerea was tested in vitro. Spore germination of B. cinerea was strongly inhibited in the presence of JA cell suspension. Furthermore, B. subtilis JA can produce antifungal volatiles which strongly inhibited the spore germination and mycelial growth of B. cinerea. As a biocontrol agent, the synergic effect of lipopeptides and volatiles may play a major role in controlling the pathogens by B. subtilis JA.

关 键 词：枯草芽孢杆菌 脂肽类抗生素 挥发性抑菌物质 

分 类 号：S476[农业科学—农业昆虫与害虫防治]