一株高耐氧反硝化细菌的筛选及其反硝化产物确定  

An Oxygen-tolerant Denitrifying Strain and Its Denitrifying Processes

作　　者：何芳[1] 康贻军[1] 单君[1] 胡健[1] 殷士学[1] 

机构地区：[1]扬州大学环境科学与工程学院,扬州225009

出　　处：《微生物学通报》2008年第1期35-39,共5页Microbiology China

基　　金：国家自然科学基金资助项目(No.30470060和No.40471072)

摘　　要：利用浅层振荡培养和连续通气培养方法,获得一株高耐氧反硝化细菌H1。分别利用NO报告克隆nntS-gfp和乙炔抑制-气相色谱测得菌株H1能够在反硝化条件下产生NO和N_2O,不能产生N_2,因此其反硝化途径为NO_3^-→NO_2^-→NO→N_2O。在初始O_2浓度为0%～21%范围内,该菌株能将98%以上的NO_3^-还原为气态氮化物。在150 mL的培养液中,连续以2 L/min的速率通气,H1依然能够反硝化,但是更高的通气速率则反硝化停止。16S rDNA序列分析表明,菌株H1与Ralstonia taiwanensis相似性达98%。An oxygen-tolerant denitrifying strain designated as H1 was screened by the procedures of shallow shaking and continuous aeration cultures. With the aid of an nnrS-gfp fusion responsive to nitric oxide （NO） and acetylene inhibition-GC procedure, it was shown that strain HI was able to produce NO and N2O but not N2 under denitrifying conditions. Denitrifying processes were thus determined as NO3^-→NO2^-→NO-→N2O, with N2O as the end product. Strain H1 could denitfify under shallow shaking conditions as well as in the initial atmospheric oxygen concentration ranging from 0-21%. Denitrification processed normally under continuous aeration at the rate of 2 L air per min in a 150 mL medium, but stopped under high aeration rate as 5 L air per min. 16S rRNA gene sequence revealed that strain HI shared 98% similarity to its closet relative Ralstonia taiwanensis, the genus where denitrifying bacteria are frequently found.

关 键 词：反硝化菌 反硝化产物 筛选 耐氧 

分 类 号：Q93[生物学—微生物学]