溶藻弧菌HY9901 AcrA基因克隆与序列分析(英文)  

Characterization of AcrA gene from Vibrio alginolyticus Strain HY9901

作　　者：蔡双虎[1,2,3] 吴灶和[1,2,3] 简纪常[1,2,3] 鲁义善[1,2,3] 

机构地区：[1]广东海洋大学水产学院,湛江524025 [2]广东省水产经济动物病原生物学及流行病学重点实验室,湛江524025 [3]广东省水产经济动物病害控制重点实验室,湛江524025

出　　处：《微生物学通报》2008年第1期67-72,共6页Microbiology China

基　　金：Guangdong Natural Science Foundation(No.011812);Guangdong Municipal Science and Technology Project (No.A3050302)

摘　　要：Touchdown PCR扩增溶藻弧菌HY9901 AcrA基因部分序列,得一460 bp片段,再以反向PCR和巢式PCR联合扩增其侧翼序列,拼接得一由1101 nt组成,共编码366 aa的完整基因。该基因演绎的氨基酸序列与几种弧菌的同源性都比较高,与创伤弧菌YJ016、副溶血弧菌RIMD 2210633、灿烂弧菌12B01、霍乱弧菌O1 N16961同源性分别为76%、73%、71%和70%。A 460 bp internal fragment of the AcrA gene from Vibrio alginolyticus strain HY9901 was amplified by PCR with designed primers and the unknown flanking sequence of 5 ′- and 3′- ends of the AcrA gene was finally characterized by inverse PCR and nested PCR. Sequence analysis showed the AcrA gene contained 1101 bp ORF encoding 366 amino acids and the deduced amino acid sequence of the precursor from Vibrio alginolyticus strain HY9901 showed significant homology with the putative protein of other Vibrio species. The AcrA shows 76%, 73%, 71% and 70% homology with V. vulnificus strain YJ016, V. parahaemolyticus strain RIMD 2210633, V. splendidus strain 12B01 and V. cholerae O1 biovar eltor str. N 16961 respectively.

关 键 词：溶藻弧菌 AcrA基因 基因克隆 

分 类 号：Q93[生物学—微生物学]