n-3系列多不饱和脂肪酸(PUFA)富集保健功能蛋开发研究  

Development of Rich n-3PUFA Egg with Health-care Function by Using Biological Resources

作　　者：潘学燕[1] 高玉鹏[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农业学报》2008年第1期20-26,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省科技攻关项目"粉壳蛋高效杂交生产及保健功能蛋研制关键技术应用项目"部分内容(编号:2004K02-G3-01)

摘　　要：以胡麻籽、紫苏籽作为α-亚麻酸资源主要来源合理配合组成6个配方，以茶叶，辅配杜仲叶、银杏叶、沙棘嫩枝叶等有效配合，制成复合粉剂，作为抗氧化剂添加剂，研究生产富含n-3PUFA的功能蛋。选用35周龄尼克粉蛋鸡945只，随机等分为7组，每组3个重复，每重复45只，其中1～6组为试验组，分别添加6个试验配方和抗氧化复方剂或vE或未加抗氧化剂，饲喂结果表明：①以胡麻籽、紫苏籽作为n-3PUFA饲源生产富集n-3PUFA功能蛋时，试验30d蛋品脂肪酸组成趋于稳定，且随着日粮PUFA水平的提高n-3PUFA富集效应越明显，特别是富集了EPA和DHA，蛋品中的n-3PUFA增加了2．7～4．1倍，EPA由未检出增加到占蛋鸡脂肪酸的0．16％～0．24％，DHA由占脂肪酸的0．57％～0．66％增加到1．94％～2．64％。蛋品中n-6PUFA／n-3PUFA比值由13～15：1降至4～5：1，符合保健膳食结构要求。②用胡麻籽、紫苏籽作日粮n-3PUFA源饲喂蛋鸡，在鸡蛋中富集n-3PUFA的同时，鸡肉脂肪酸的组成亦发生变化，其总趋势与蛋品相似，即n-3PUFA在鸡肉中亦得到富集，其中胸肌中n-3PUFA由占脂肪酸的3．94％增加到7．22％～9．42％。③用胡麻籽、紫苏籽作日粮n-3PUFA源饲喂蛋鸡生产富集n-3PUFA功能蛋时，蛋品的脂质过氧化物水平增加，其中饲喂30d后，丙二醛（MDA）由82．54μmol／g增加到192．44μmol／g，但添加茶叶等生物抗氧化剂后，则降低为53．25～79．45μmol／g，效果显著，优于添加vE的抗氧化效果。945 Coral Nick laying hens with age of 35 weeks are chosen and splitted into 7 groups at random, there are 3 repetitions in one group, every repetition has 45 hens, the main source of α-Linolenic acid is from benne seeds and the seeds of Perilla frutescens （L.） Britt, the compound power is made by adding leaves of Eucommia ulmoides and Ginkgo and fresh leaves and of seabuckthern to anti oxidization, the functional eggs which are produced are rich in n-3PUFA, the results indicated：（1） when benne seeds and the seeds of Perilla frutescens （L.） Britt are taken as the feeding source of n-3PUFA for produce n-3PUFA functional eggs, after 30 days＇ experiment, the fatty acid component of eggs is stable, the concentration effect of n-3PUFA is also remarkable with the increase of PUFA diet, especially it concentrates EPA and DHA greatly, the ration of n-6 to n-3 decreases from 13-15 ： 1 to 4-5 ： 1, this can meet requirement of health-care diet structure. （2） if benne seeds and the seeds of Perilla frutescens （L.） Britt are taken as source of n-3PUFA diet to feed laying hens, the fatty acid structure in hens＇ meat changes, at the same time,the eggs are rich in n-3PUFA,the main trendency are similar to eggs products, that is n-3PUFA is getting more in hens＇ meat. （3） when benne seeds and the seeds of PeriZZa frutescens （L.） Britt are taken as source of n-3PUFA diet to produce functional eggs rich in n-3PUFA, the main indexes of eggs have not been affected, but if more amounts are added, the production performance of laying hens are affected. Adding tea power can control the by-effect greatly. The experiment which the tea power is taken as main raw materials of anti-oxidation is better than rE, even more; it can decrease the MDA of the eggs.

关 键 词：生物资源 保健功能蛋 n-3PUFA富集 n-3PUFA/n-6PUFA比例 

分 类 号：Q51[生物学—生物化学]