侵染荷兰郁金香的郁金香碎色病毒和黄瓜花叶病毒的RT-PCR检测  

Detection of Tulip Breaking Virus and Cucumber Mosaic Virus in Four Tulip Cultivars from Holland by RT-PCR

作　　者：杨玉秀[1] 王海妮[2] 吴云锋[2] 

机构地区：[1]西安植物园,西安710061 [2]西北农林科技大学植保学院,陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《西北农业学报》2008年第1期113-115,共3页Acta Agriculturae Boreali-occidentalis Sinica

摘　　要：以从荷兰引进的4个郁金香品种病株为材料,利用马铃薯Y病毒的简并引物和黄瓜花叶病毒CP基因的一对特异引物,通过RT-PCR技术分别扩增出与预期大小相一致的1800 bp和840 bp左右的片段,而对照无任何产物。感受态转化后,进行测序,结果表明田野、多道多、雪莉和克思尼4个品种中均能检测到郁金香碎色病毒和黄瓜花叶病毒。Four tulip cultivars were introduced from Holland. Degenerate primers for potato Y virus and specific primer for cp gene of cucumber mosaic virus was designed , The 1800bp and 840bp DNA fragement was amplified in the four tulip cultivars by the reverse transcription and polymerase chain reaction （RT-PCR）, while no fragment was obtained from the control . The sequence result revealed that both tulip breaking virus and cucumber mosaic virus were detected from Tianye, Duodaoduo, Xueli, Kesini respectively.

关 键 词：郁金香 郁金香碎色病毒 黄瓜花叶病毒 RT-PCR 检测 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]