大口径毛细管气相色谱分离和测定银杏叶萜内酯的方法建立  

Separation and Determination of Terpene Trilactones by Gas Chromatography with Wide Bore Capillary Column

作　　者：廖咏玲[1] 许锋[1] 朱俊[1] 王燕[1] 程水源[1] 

机构地区：[1]长江大学园艺园林学院,湖北荆州434025

出　　处：《西北农业学报》2008年第1期146-149,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：湖北省重大科技攻关项目(2004AA204B03);荆州市科技发展项目(20041PB06);湖北省自然科学基金(2006ABA005)

摘　　要：为建立灵敏、简便测定银杏叶萜内酯气相色谱法，试验采用大口径毛细管气相色谱法，对银杏叶中的银杏内酯A（GA）、银杏内酯B（GB）、银杏内酯C（GC）和白果内酯（BB）4种银杏萜内酯进行了分离与测定。用20％的甲醇水溶液提取银杏叶中的银杏内酯A，B，C和白果内酯，乙酸乙酯（EOA）／四氢呋喃（THF）一步纯化，衍生后进行GC-FID检测。样品在16min内完全分离，GA、GB、GC和BB的相对标准偏差均小于0．8％，平均回收率为94．45％～98．98％。本方法测定银杏叶萜内酯简便、灵敏、准确、重现性好。In order to determined terpene trilactones simplely and rapidly from Ginkgo biloba leaves. A method for the simultaneous separation and determination of terpene trilactones from Ginkgo biloba leaf by gas chromatography with wide-bore capillary column was described. The results showed that the components were extracted from Ginkgo biloba leaves with 20% methanol in water, the extracts were dissolved in 10% NaH2PO4 solution under ultrasonication and separated from the solution by extraction with a mixture of ethyl acetate/tetrahdrofuran in a capped vial. After derivatization, these terpene trilactones could be quantified and qualitative analyzed using GC-FID. All components were separated in 16min. The relative standard deviations were less than 0.8%, and the average recoveries were 94.45%-98.98%. The method is simple, rapid, sensitive and reproducible.

关 键 词：大口径毛细管 气相色谱法 银杏萜内酯 提取 检测 

分 类 号：R284[医药卫生—中药学]