新疆雪莲多倍体的诱导初探  

Polyploid Induction of Saussurea involucrata Kar. et Kir.

作　　者：张蜀敏[1] 王晓军[1] 郝秀英[2] 徐琴[1] 陈阳[1] 刘传军[1] 曹玉锦[1] 欧阳立恒[2] 

机构地区：[1]中国科学院新疆理化技术研究所 [2]新疆农业科学院微生物应用研究所,乌鲁木齐830091

出　　处：《西北农业学报》2008年第1期216-220,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：中国科学院西部行动高新技术项目:新疆雪莲规模化组培快繁技术研究(KGCX2-SW-506)

摘　　要：用秋水仙素和2%二甲基亚砜共同处理新疆雪莲种子、胚根和茎段,以诱导多倍体雪莲。结果表明,茎段经过100 mg.L-1秋水仙素处理3 d诱导效果最佳,同时,离体培养其嵌合体苗茎段及叶片,诱导不定芽生成,获得了四倍体细胞比例达到90%以上的植株。The stem,seed,radicel of Saussurea involucrata Kar. et Kir. were induced with colchicine and 2%DMSO. The results showed that the better effective induction team is that the stem was induced with 100 mg · L^-1 colchicine for 3 d. Furthermore, the stem and leaf of the mixoploid of the 100 mg · L^-1 colchicine were cultivated for 3 d to stimulate the adventitious buds to regenerate and the plants with more than 90% of tetrapolid cell were gained.

关 键 词：秋水仙素 新疆雪莲 多倍体 

分 类 号：S567.239[农业科学—中草药栽培]