光接枝表面修饰法制备牛血红蛋白的分子印迹微球  

Preparation of Bovine Hemoglobin-imprinted Polymer Beads via Photografting Surface-Modified Method

作　　者：盖青青[1] 刘秋叶[1] 李文友[1] 何锡文[1] 陈朗星[1] 张玉奎[1] 

机构地区：[1]南开大学化学系

出　　处：《高等学校化学学报》2008年第1期64-70,共7页Chemical Journal of Chinese Universities

基　　金：国家“九七三”计划(批准号:2007CB914101);国家自然科学基金(批准号:20675040);天津市自然科学基金(批准号:06YFJMJC02800,07JCYBJC00500)资助

摘　　要：聚苯乙烯球载体表面经引发转移终止剂修饰后,采用光接枝表面印迹方法制备了以牛血红蛋白(BHb)为模板分子、丙烯酰胺为功能单体和N,N′-亚甲基双丙烯酰胺为交联剂的分子印迹聚合物微球(MIP).进一步采用红外光谱(IR)、扫描电子显微镜(SEM)和元素分析对聚合物微球进行了表征,证实了载体表面成功地接枝了分子印迹层,并研究了其吸附性能和分子识别选择性能.结果表明,采用光接枝表面修饰法制备的分子印迹微球对模板分子有着很好的吸附容量和识别选择性.Molecularly imprinted polymers （MIP） based on photografting surface-modified polystyrene beads as matrices were prepared with aerylamide as the functional monomer, bovine hemoglobin as the template molecule and N, N′-methylene bisaerylamide as the erosslinker in a phosphate buffer. The results of IR, scanning electron microscope （SEM） and elemental analysis demonstrate the formation of a grafting polymer layer on the polystyrene-bead surface. Subsequent removal of the template leaves behind cavities on the surface of polymer matrix with a shape and an arrangement of functional groups in complementary binding sites to the original template. And the adsorption studies show that the imprinted polymers have a good adsorption capacity and specific recognition for bovine hemoglobin as the template molecule. Our results demonstrated that the polymer prepared via photografting surface-modified method exhibited a better selectivity for the template. Attempt to employ the new method in molecular imprinting techniques may indroduee new application to MIPs and faeilicate the probable protein separation and purification.

关 键 词：分子印迹 分子识别 牛血红蛋白 光接枝 引发转移终止剂 

分 类 号：O657.32[理学—分析化学] O643[理学—化学]