陕西省农家种大豆资源11S和7S球蛋白含量分析  

The analysis of 7S and 11S globulin content of soybean landraces in Shaanxi

作　　者：张敏娟[1] 刘萌娟[2] 赵惠贤[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]西北农林科技大学农学院,陕西杨凌712100

出　　处：《干旱地区农业研究》2008年第1期187-193,共7页Agricultural Research in the Arid Areas

基　　金：农业部种质资源保护项目(NB04-22-6);西北农林科技大学青年学术骨干支持计划

摘　　要：利用SDS—PAGE分析了陕西省75份农家种大豆核心种质的11S和7S球蛋白亚基组成、相对百分含量和11S／7S比值，并对陕北、关中和陕南三个地区的资源材料进行了分析和比较。结果表明：大豆贮藏蛋白质由许多亚基组成，其中含量较高的有α＇、α、β、A3、Acid和Basic；不同品种间大豆球蛋白组成几乎没有差异，但是各亚基的含量存在较大差异；75份供试材料的7S、11S球蛋白含量的平均值分别为36％和64％，交幅分别为28％～48％和52％～72％；11S／7S比值的交异幅度在1．09～2．53，平均值为1．78，高于2．0的种质有19个；陕北、关中、陕南三个地区农家种大豆群体11S／7S比值存在显著差异。The relative percentage content of 11S and 7S and the 11S/7s value of 75 soybean landraces from Shaanxi province were investigated by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE） in this study. The results showed that, soybean storage protein have various subunits, in which α＇, α, β, A3, Acid and Basic have a high content; Although the band type of soybean storage protein are basically the same, the content among different subunits of each landrace is significant difference, llS and 7S globulin average content were 36% and 64%, respectively, their variational range were from 28% to 48% and from 52% to 72%, respectively, 11S/7S value ranged from 1.09 to 2.53, with an average of 1.78; 11S/7S value was significantly different among the three cu.ltivated regions.

关 键 词：大豆 农家种 球蛋白 SDS—PAGE 

分 类 号：S565.1[农业科学—作物学]