固相萃取-顺序注射分光光度法快速测定微生物菌落中的麦白霉素  

Rapid spectrophotometric quantification of meleumycin in colonies of streptomyces mycarofaciens via solid phase extraction in a sequential injection system

作　　者：舒杨[1] 陈旭伟[1] 王建华[1] 

机构地区：[1]东北大学分析科学研究中心

出　　处：《分析试验室》2008年第1期13-15,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20575010,20635010);教育部博士点基金(20050145026)项目资助

摘　　要：在顺序注射系统中引入C18固相萃取柱进行生米卡链霉菌菌落样品的纯化富集，建立了快速测定菌落中麦白霉素的分光光度法。用超声波破碎菌落细胞后，将样品溶液通过固相萃取柱，用少量甲醇洗脱吸附在C18微珠上的麦白霉素，232am检测。选样量为2ml，洗脱液体积为100μL，麦白霉素的富集倍率为14；线性范围为0．1～10μg／mL，检出限为0．02μg／mL，RSD为2．5％（0．5μg／mL，n=7）。菌落样品中麦白霉素的加标回收率为96％～100％。A rapid spectrophotometric method for the determination of meleumycin in colonies of streptomyces mycarofaciens was developed via solid-phase extraction in a sequential injection system. After meleumycin was released from the cell by ultrasonic lysis, 2.0 mL of sample solution was directed to flow through a C18 packed mini-column. The meleumycin retained onto the column was afterwards eluted with 100μL of methanol and the eluate was monitored at a wavelength of 232 nm. With a 2 mL sampling volume, an enrichment factor of 14 was achieved within 0.1 - 10 μg/mL, and a detection limit of 0.02 μg/mL was derived along with a RSD of 2.5 % at 0.50μg/L. Spiking recoveries of 96% - 100% for meleumycin in three colony samples were obtained.

关 键 词：麦白霉素 顺序注射 固相萃取 分光光度法 

分 类 号：O657.3[理学—分析化学]