薄层色谱-紫外可见分光光度法测定食品中的苏丹红Ⅰ号  

Determination of Sudan red Ⅰ in food by TLC-UV-Vis spectrophotometry

作　　者：庞艳玲[1] 王怀友[2] 

机构地区：[1]菏泽学院化学与化工系,菏泽274015 [2]山东师范大学化学化工与材料科学学院,济南250014

出　　处：《分析试验室》2008年第1期60-62,共3页Chinese Journal of Analysis Laboratory

基　　金：山东省自然科学基金(Y2006B31)项目资助

摘　　要：建立了测定食品中苏丹红Ⅰ号新方法：薄层色谱-紫外可见分光光度法。方法的线性回归方程为：A=0．00583＋0．15105,0，在0．50～10．0μg／mL之间苏丹红Ⅰ号的浓度与吸光度之间呈线性关系，相关系数R=0．9992，检出限为0．05μg／mL。测定结果与国家标准HPLC法对照，令人满意。可用于食品中苏丹红Ⅰ号的测定。A new method for the determination of Sudan red I in food by TLC-UV-Vis spectrophotometry was established. The linear regression equation, A = 0.00583 ＋ 0. 15105C, with a linear correlation coefficient of 0.9992, was obtained. The linear relationship was found between the absorbance and the concentration of sudan red Ⅰ in the rang of 0.50 - 10.0μg/mL. The detection limit was 0.05μg/mL. The results obtained by this method agreed with that of official method. This method was applied successfully to determination of Sudan red Ⅰ with satisfactory results.

关 键 词：苏丹红 薄层分离 紫外可见分光光度法 超声萃取 

分 类 号：O657.3[理学—分析化学]