饮酒者唾液中乙醇浓度的酶催化光度法测定  

Catalytic spectrophotometry with enzyme for determination of ethanol in saliva of drinker

作　　者：廖丽霞[1] 宋丹丹[2] 方涛[1] 许汉英[2] 

机构地区：[1]东北林业大学理学院,哈尔滨150040 [2]华中师范大学化学学院,武汉430079

出　　处：《分析试验室》2008年第1期70-72,共3页Chinese Journal of Analysis Laboratory

基　　金：湖北省自然科学基金重点攻关(992P0315)项目资助

摘　　要：研究了醇氧化酶-过氧化氢酶联合的酶促反应的最佳条件，建立了简便测定乙醇的吸光光度法，乙醇浓度在0．0686～5．115mmol／L范围内符合比尔定律，回归方程为：A=0．00403＋0．08585c（mmol／L），相关系数为0．9986，检出限为0．0172mmol／L（S／N=3），本法所用仪器简单，已用于酒后唾液中乙醇的测定。The optimum conditions for alcohol oxidase and catalase catalytic system were studied. The spectrophotometric method for determining ethanol was established. The linear range of ethanol was found to be 0.0686 ～ 5. 115 mmol/L with a correlation coefficient of 0.9986, and the regression equation is A = 0.00403 ＋ 0.08585 c. The detection limit of this method is 0.0172 mmol/L. The method has merits of simplicity, convenience and selectivity, and hence can be used for the determination of ethanol in saliva of drinkers with satisfactory results.

关 键 词：酶催化光度法 乙醇 唾液 

分 类 号：O657.32[理学—分析化学]