两株高致病性PRRSV ORF6和ORF7基因克隆及序列分析  

Clone and Sequence Analysis of Two Highly Pathogenic PRRSV ORF6 and ORF7 Genes

作　　者：时建立[1] 李俊[2] 徐绍建[1] 赵鸿雁[1] 张秀美[2] 王金宝[2] 

机构地区：[1]青岛农业大学,青岛266109 [2]山东省农科院畜牧兽医研究所,济南250100

出　　处：《中国畜牧兽医》2008年第1期39-43,共5页China Animal Husbandry & Veterinary Medicine

基　　金：山东省自然基金重大项目(Z2007D06)

摘　　要：应用RT—PCR方法从实验室分离的两株高致病性PRRSV SX、ZQ株中扩增出ORF6和ORF7，将其分别克隆、测序。用DNAStar软件分析所测序列，并与VR-2332株、LV株、周边国家及国内分离株进行核苷酸和推导氨基酸同源性比较，并绘制系统进化树，结果ORF6、ORF7核苷酸与北美洲型的同源性为91．1％～100％，与欧洲型的同源性为66．2％～70．7％，推导氨基酸与北美洲型的同源性为91．2％～100％，与欧洲型的同源性为62．3％～82．3％。证明新分离到的PRRSVSX株、ZQ株仍属北美洲型。SX株ORF6、ORF7核苷酸与国内新分离到的高致病性PRRSV JXA1株同源性分别为99．8％、100％；ZQ株ORF6、ORF7核苷酸与国内新分离到的高致病性PRRSV JXA1株同源性分别为99．6％、99．7％。The ORF6 and ORF7 genes of two highly pathogenic PRRSV SX, ZQ, derived from Shandong,were amplified by RT-PCR. The products were sequenced after cloning into pMD18-T vector respectively . The sequences were analysed using DNAStar and compared with those of VR- 2332, LV and other strains isolated from China and circumjacent Country. The homologous of ORF6,ORF7 Nuclear acid was 91.1% - 100 % with North American（VR-2332） and 66.2 %- 70.7 % with European（LV） viruses, The homologous of ORF6,ORF7 deduced amino acid was 91.2%-100% with North American（VR-2332） and 62.3 % -82.3% with European（LV） viruses,indicating that the two new shandong isolates were related to the North A- merican PRRSV genotype. The ORF6, ORF7 Nuclear acid of SX shared 99.8%, 100% identity with highly pathogenic PRRSV JXA1, while The ORF6, ORF7 Nuclear acid of ZQ shared 99.6%, 99.7 % identity with highly pathogenic PRRSV JXA1.

关 键 词：高致病性PRRSV ORF6 ORF7 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]