CYP1A1基因多态性与妊娠期肝内胆汁淤积症相关性研究  

Association between CYP1A1 gene polymorphism and intrahepatic cholestasis of pregnancy

作　　者：王晓莉[1] 张力[2] 欧容清[1] 王璟[1] 刘淑芸[2] 陈强[3] 苏品璨[2] 

机构地区：[1]成都市第二人民医院妇产科,610017 [2]四川大学华西第二医院妇产科 [3]四川省脐带血造血干细胞库

出　　处：《中华医学遗传学杂志》2008年第1期70-72,共3页Chinese Journal of Medical Genetics

基　　金：成都市卫生局科研基金（05005）

摘　　要：目的探讨参与雌激素代谢的细胞色素P4501A1 （cytochrome P450 1A1, CYP1A1 ）基因多态性与成都地区妊娠期肝内胆汁淤积症（ intrahepatic cholestasis of pregnancy, ICP） 的关系。方法分别应用聚合酶链反应．限制性片段长度多态性技术和等位基因特异性PCR技术，对100例ICP患者和100名正常对照孕妇CYP1A1基因Msp Ⅰ位点和He／Val位点多态性进行分析。结果MspⅠ位点多态性在ICP组和对照组中的分布差异无统计学意义（P〉0．05），而ICP组含Val等位基因的Ile／VM和Val／Val基因型增加ICP的发病风险（P=0．047，OR=1．768）。结论CYP1A1基因第7外显子的Ile／VM基因多态性可能与成都地区ICP易感性有关；而Msp Ⅰ位点多态性与ICP的发生无相关性。Objective To study the relationship between CYP1A1 genetic polymorphism and intrahepatic cholestasis of pregnancy （ICP） in Chengdu of China. Melhods Msp Ⅰ and Ile/Val genotypes of CYP1A1 gene were detected with polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and allele-specific amplification-PCR （ASA-PCR） in a case-control study, including 100 cases of ICP and 100 controls. Results There was no significant correlation between Msp Ⅰ pelymorphism and ICP susceptibility （ P 〉 0.05）. However, the Ile/Val ＋ Val/ Val genotypes of CYP1A1 significantly increased the risk of ICP （ P = 0.047, OR = 1.768）. Conclusion The Ile/Val pelymorphism in exon 7 of CYP1A1 may be associated with the susceptibility of ICP in Chengdu. The Msp Ⅰ pelymorphism of CYP1A1 is not associated with the risk of ICP in Chengdu.

关 键 词：妊娠期肝内胆汁淤积症 细胞色素P4501A1 遗传多态性 

分 类 号：R714.2[医药卫生—妇产科学]