碳酸化羟基磷灰石骨水泥的开发与多孔化研究  

Porosity study of the carbonated hydroxyapatite cement

作　　者：唐佩福[1] 姚琦[1] 黄鹏[1] 郝立波[1] 毛克亚[1] 王岩[1] 卢世璧[1] 王继芳[1] 

机构地区：[1]解放军总医院骨科,北京复兴路100853

出　　处：《中国矫形外科杂志》2008年第2期124-126,共3页Orthopedic Journal of China

基　　金：国家自然科学基金项目(No.39670731)

摘　　要：[目的]应用无机化学材料合成碳酸化羟基磷灰石骨水泥,并通过发泡成孔的新方法制备能原位固化形成多孔结构的无机植骨材料。[方法](1)以碳酸钙、磷酸氢钙等化学试剂为原材料,通过高温烧结合成碳酸化羟基磷灰石粉体。再以磷酸氢二钠和磷酸二氢钠合成浓度为0.2mol/L的磷酸钠缓冲液,作为固化液。粉体与固化液以1g/0.4ml相混合,原位固化形成碳酸化羟基磷灰石;(2)在碳酸化羟基磷灰石粉体中加入成孔剂,成孔剂在骨水泥固化过程中发生化学反应产生二氧化碳气体,由此形成多孔碳酸化羟基磷灰石骨水泥;(3)通过化学成分、化学结构、力学强度、固化时间、孔隙率等检测,对其理化性能、可操作性能和组织相容性进行评估。[结果]所合成的碳酸化羟基磷灰石骨水泥原位固化性能好,固化时间为12～15min,可操作性能强;化学成分分析发现,其固化产物中含有5.6%的碳酸根成分,与天然骨的矿物相类似;孔隙分析表明,材料的孔隙之间相互贯通,孔隙率为36%;力学测试结果显示,材料的抗压强度为4.3±2.6MPa,相当于松质骨的力学强度;细胞毒性实验证明,该材料具有优秀的组织相容性。[结论]多孔碳酸化羟基磷灰石骨水泥的化学成分、理化性能、孔隙结构均与天然的松质骨相似,组织相容性良好,可作为无机植骨材料。[ Objective] To compose carbonated hydroxyapatite cement with chemica 1 materials, and by adding the pore agent to develop a new bone substitute, which can be solidified in situ to form porous carbonated hydroxyapatite. [ Method] （ 1 ）A new type of carbonated hydroxyapatite cement （CHC） was prepared. The powder of cement was composed of calcium carbonate, tricalcium phosphate and calcium phosphate dibasic. The liquids were prepared by 0.2mol/L sodium phosphate buffer, solide phase to liquid phase was lg to 0.4 ml; （2）To prepare an in situ setting porous carbonated hydroxyapatite cement,add the pore agent into the powder of cement, pore generate CO2 during situ setting of cement; （ 3 ） The chemcial composition, chemcial constitution mechanical property, setting time , interval porosity of the PCHA were tested, and then the physio-chemical character, manipulatity, histocompatibility were evaluated. [ Result] Addition of pore agent could succeed to prepare a new bone substitute which could set in situ and transform into porous carbonated hydroxyapatite. The setting time was 13 - 15 minutes which was suitable to clinical application. The pore size and porosity character could be controlled by adjustment of the component. The chec- king results demonstrated that the self-setting composition of this cement was carbonated hydroxyapatite which was similar with the mineral phase of natural cancellous bone,the carbonic acid radical was 5.6% in the solidify production. Contain of the porosity was 36% with interconnect pore, the compressive strength was 5.6 ±2.2 MPa which was equal to strength of cancellous bone, and the cytotoxicity tests showed an exellent biocompatibility. [ Conclusion ] The porous carbonated hydroxyapatite cement is a good bone substitute which seems to be the cancellous bone with good porosities, exellent biocompatibility.

关 键 词：碳酸化羟基磷灰石 骨水泥 多孔 原位固化 

分 类 号：R318.08[医药卫生—生物医学工程]