红千层愈伤组织诱导及植株再生  

Callus Induction and Plant Regeneration in Callistemon rigidus

作　　者：伍成厚[1] 冯毅敏[1] 叶振华[1] 朱纯[1] 龙丽萍[1] 

机构地区：[1]广州市园林科学研究所

出　　处：《中南林业科技大学学报》2007年第6期104-108,共5页Journal of Central South University of Forestry & Technology

基　　金：广东省科技计划项目(2004B60302006);广州市科技计划项目(004Z3-E0361)的部分研究内容

摘　　要：以茎段、芽和叶片为材料,探讨了红千层愈伤组织诱导及植株再生的方法.结果表明:红千层的茎段、芽和叶片均可诱导出愈伤组织,通过继代培养可发育成绿色和粉红色2种类型的愈伤组织,其中绿色、致密的愈伤组织可以分化出不定芽;培养基1/2M S+6-BA 1.0 m g/L+NAA 0.1 m g/L适宜愈伤组织不定芽的诱导,在培养基1/2M S+IBA 0.25 m g/L上试管苗的生根率可达95%.Taking stem segment,bud and leaf as materials,this paper made a study of callus induction and plant regeneration in Callistemon rigidus R.Br.The results show that callus can be induced from all types of materials and after being sub-cultured,the callus will develop to two kinds of colors that are pink and green;that the pink callus will be degenerated while the compact green one will induce adventitious buds.The optimal medium for adventitious bud inducing is 1/2 MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/L and the optimal for the rooting of test-tube plantlets is 1/2MS＋IBA0.25 mg/L on which its rate can reach 95 % after being cultured for 40 days.

关 键 词：红千层 愈伤组织诱导 植株再生 组织培养 

分 类 号：S685.99[农业科学—观赏园艺] Q945.52[农业科学—园艺学]