薄层色谱定性示波极谱定量测定苏丹红(Ⅰ、Ⅱ)的方法研究  

Qualitative Analysis of SudanⅠand SudanⅡ in Foods with Thin Layer Chromatography and Quantitative Detection of Them with Polarography.

作　　者：陈宇 李芳[2] 刘兆霖 冉燕 聂忠莉 杨林 曹东梅 

机构地区：[1]富顺县疾病预防控制中心,四川自贡643200 [2]自贡市第四人民医院,四川自贡643000 [3]自贡市疾病预防控制中心,四川自贡643000 [4]富顺县保健院,四川自贡643200 [5]自贡药品监督检验所,四川自贡643000

出　　处：《四川省卫生管理干部学院学报》2007年第4期249-252,共4页Journal of Sichuan Continuing Education College of Medical Sciences

摘　　要：目的：建立食品和药品中苏丹红的示波极谱测定方法。方法：样品经过前处理除去干扰杂质后，用三氯甲烷溶解定容；在聚酰胺薄层板上点样，于甲醇-丙酮-乙酸（60：20：20，V／V）展开剂中层析分离，与标准物Rf值比较定性；分离物于7．38％乙酸钠和24．8％乙酸底液中极谱扫描定量测定苏丹红Ⅰ和苏丹红Ⅱ。结果：苏丹红Ⅰ和苏丹红Ⅱ均在0～90μg／10ml呈线性关系（r〉0．999），最低检出限0．2μg/ml；对辣椒粉、香辣酱和药品进行加标回收测定，苏丹红Ⅰ收回率95．2％～109．9％，苏丹红Ⅱ收回率92．5％～109．3％，相对标准差（n=6）小于5％，用该法和国家标准方法同时测定9件样品，测定结果经统计学分析，两法结果无显著性差异（苏丹红Ⅰ：t=0．704，P〉0．05；苏丹红Ⅱ：t=0．0318，P〉0．05）。结论：建立的方法操作简单，重现性好，灵敏度高，结果准确，适于基层单位应用。Objectives： To set up Polarngraphy detection method of Sudan Ⅰ and SudanⅡ in food and drug. Methods： The impurity of a sample was conducted by previous treating, then dissolved and fixed vohanes with chloroform. We took the sample in the slight layer of polyamida, and dispersed in methanol and acetone and acetic acid （60：20：20, V/V） . Determining Rf values were contrasted to references; On the condition of 7.38 % acetic acid sodium- 24.8% acetic acid, to build up Polarography to measure Sudan Ⅰ and Sudan Ⅱ in food and drugs. Results： The standard series presented the rectilinear relation （ r 〉 0.999） within the Sudan Ⅰ and SudanⅡ of 0 90μg/ml, the minimum detected concentration was 0.2μg/ml. The different concentrations of Sudan Ⅰ and Sudan Ⅱ standard things were joined into the red pepper and in the red pepper and thick chilli paste and drug and were measured 6 times. The recovery rate for Sudan Ⅰ were 95.2% - 109.9%and Sudan Ⅱ 92.5% - 109.3% .The relative standard deviation （ n = 6） was less than 5%. The nine samples were measured with this method and the national standard GB/T19681-2005. There were no significant differences （ Sudan Ⅰ ： t = 0.704, P 〉 0.05 ; Sudan Ⅱ ： t = 0.0318, P 〉 0.05） . Conclusions： The operation is simple and fast. The result is accurate and the method is worth applying.

关 键 词：薄层色谱法 示波极谱法 食品 药品 苏丹红Ⅰ 苏丹红Ⅱ 

分 类 号：R657.14[医药卫生—外科学]