Apelin-13刺激大鼠血管平滑肌细胞增殖  

Apelin-13 stimulation of VSMCs proliferation in rats

作　　者：李峰[1] 李兰芳[1] 秦旭平[1] 潘伟男[1] 朱炳阳[1] 廖端芳[1] 陈临溪[1] 

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001

出　　处：《基础医学与临床》2008年第1期9-12,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30371645);湖南省教育厅优秀青年基金(03B036)

摘　　要：目的研究G蛋白耦联受体APJ的内源性配体多肽apelin-13对大鼠血管平滑肌细胞(VSMCs)增殖的影响及其与ERK1/2信号通路的关系。方法采用噻唑蓝比色法(MTT)、Western blot等方法观察apelin-13对体外培养大鼠VSMCs增殖的影响及细胞周期蛋白表达的变化。结果Apelin-13浓度依赖性促进VSMCs增殖,且cyclin D1表达增加;ERK1/2阻断剂PD98059可明显抑制apelin-13诱导的细胞增殖及pERK1/2、cyclin D1表达。结论Apelin-13能促进大鼠VSMCs增殖,其机制可能与apelin/APJ-ERK1/2-cyclin D1信号通路有关。Objective To observe the effect of G protein-coupled receptor APJ endogenous ligand apelin-13 on the proliferation of vascular smooth muscle cells （ VSMCs）, and to investigate whether the effect is related to apelin/ APJ-pERK1/2-cyclin D1 signal transduction pathway. Methods Cell proliferation was measured by MTT assay and the expression of cyclin D1 and pERK1/2 were determined by Western blot. Results The rusults showed that apelin-13 can stimulate VSMC proliferation in a concentration-dependent manner. Western blot analysis shows that apelin-13 stimulates the expression of cyclin D1 and pERK1/2. The ERK1/2 inhibitor PD98059 decreases the expression of pERK1/2 induced by apelin-13. Similarly, PD98059 partially diminishes the expression of cyclin D1, and inhibites the VSMCs proliferation stimulated by apelin-13. Conclusion Apelin-13 can stimulate the proliferation of VSMC and the effect is potentially mediated by apelin/APJ-pERK1/2-cyclin D1 signal cascades.

关 键 词：APELIN APJ 血管平滑肌细胞 细胞周期蛋白 ERK1/2 

分 类 号：R33[医药卫生—人体生理学]